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Table of Contents

February 2025

Cover

Wnt5a Expression in Juvenile Liver Fluke
Issue Image

Wholemount fluorescent in situ hybridisation image showing the localisation of the expression of WNT5A transcripts (red) in the mid forebody and anterior end of juvenile Fasciola hepatica. Green denotes EdU (5-ethynyl-2′-deoxyuridine (EdU) staining of neoblast-like stem cells. Worms were counterstained with DAPI (blue; 4′,6-diamidino-2-phenylindole (DAPI, blue). Scale = 50 µm. Armstrong et al

Image Credit: Rebecca Armstrong

Pearls

Decoding Cryptococcus: From African biodiversity to worldwide prevalence

Marco A. Coelho, Márcia David-Palma, Janneke Aylward, Nam Q. Pham, Cobus M. Visagie, Taygen Fuchs, Neriman Yilmaz, Francois Roets, Sheng Sun, John W. Taylor, Brenda D. Wingfield, Matthew C. Fisher, Michael J. Wingfield, Joseph Heitman

PLOS Pathogens: published February 3, 2025 | https://doi.org/10.1371/journal.ppat.1012876

Sexual recombination in cereal rust fungi: Knowns and unknowns of pathogen evolution and adaptation

Shideh Mojerlou, Benjamin Schwessinger, Julian Rodriguez-Algaba

PLOS Pathogens: published February 4, 2025 | https://doi.org/10.1371/journal.ppat.1012908

Imprudent use of MalAvi names biases the estimation of parasite diversity of avian haemosporidians

Juliana Tamayo-Quintero, Josué Martínez-de la Puente, Nubia E. Matta, M. Andreína Pacheco, Héctor F. Rivera-Gutierrez

PLOS Pathogens: published February 5, 2025 | https://doi.org/10.1371/journal.ppat.1012911

Moving beyond multi-triazole to multi-fungicide resistance: Broader selection of drug resistance in the human fungal pathogen Aspergillus fumigatus

Spyros G. Kanellopoulos, Eveline Snelders

PLOS Pathogens: published February 10, 2025 | https://doi.org/10.1371/journal.ppat.1012851

Mechanisms of resistance against allylamine and azole antifungals in Trichophyton: A renewed call for innovative molecular diagnostics in susceptibility testing

Aditya K. Gupta, Tong Wang, Avantika Mann, Vincent Piguet, Anuradha Chowdhary, Wayne L. Bakotic

PLOS Pathogens: published February 11, 2025 | https://doi.org/10.1371/journal.ppat.1012913

Understanding Mycobacterium tuberculosis through its genomic diversity and evolution

Mollie I. Sweeney, Carson E. Carranza, David M. Tobin

PLOS Pathogens: published February 28, 2025 | https://doi.org/10.1371/journal.ppat.1012956

Review

Interplay between porin deficiency, fitness, and virulence in carbapenem-non-susceptible Pseudomonas aeruginosa and Enterobacteriaceae

Hedi Mammeri, Youssouf Sereme, Eya Toumi, Hélène Faury, David Skurnik

PLOS Pathogens: published February 7, 2025 | https://doi.org/10.1371/journal.ppat.1012902

Research Articles

Iron-mediated post-transcriptional regulation in Toxoplasma gondii

Megan A. Sloan, Adam Scott, Dana Aghabi, Lucia Mrvova, Clare R. Harding

PLOS Pathogens: published February 3, 2025 | https://doi.org/10.1371/journal.ppat.1012857

Streptococcus mutans regulates ubiquitin modification of Candida albicans in the bacterial-fungal interaction

Yixin Zhang, Zhen Gu, Zhengyi Li, Qinrui Wu, Xin Xu, Xian Peng

PLOS Pathogens: published February 3, 2025 | https://doi.org/10.1371/journal.ppat.1012887

GATA1-deficient human pluripotent stem cells generate neutrophils with improved antifungal immunity that is mediated by the integrin CD18

Andrew S. Wagner, Frances M. Smith, David A. Bennin, James A. Votava, Rupsa Datta, Morgan A. Giese, Wenxuan Zhao, Melissa C. Skala, Jing Fan, Nancy P. Keller, Anna Huttenlocher

PLOS Pathogens: published February 3, 2025 | https://doi.org/10.1371/journal.ppat.1012654

Quinoxaline-based anti-schistosomal compounds have potent anti-plasmodial activity

Mukul Rawat, Gilda Padalino, Edem Adika, John Okombo, Tomas Yeo, Andrea Brancale, David A. Fidock, Karl F. Hoffmann, Marcus C. S. Lee

PLOS Pathogens: published February 3, 2025 | https://doi.org/10.1371/journal.ppat.1012216

Marek’s disease virus-1 unique gene LORF1 is involved in viral replication and MDV-1/Md5-induced atrophy of the bursa of Fabricius

Chenyi Bao, Jun Chu, Qi Gao, Shasha Yang, Xiaoyu Gao, Wenwen Chen, Fuchun Yang, Fei Jiang, Chenxi Tong, Mingyi Lei, Linlin Jiao, Jitong Li, Kexin Wei, Xue Lian, Kai Li, Suresh Kumar Tikoo, Nikolaus Osterrieder, Lorne A. Babiuk, Yufeng Li, Yong-Sam Jung, Yingjuan Qian

PLOS Pathogens: published February 3, 2025 | https://doi.org/10.1371/journal.ppat.1012891

A paradoxical population structure of var DBLα types in Africa

Mun Hua Tan, Kathryn E. Tiedje, Qian Feng, Qi Zhan, Mercedes Pascual, Heejung Shim, Yao-ban Chan, Karen P. Day

PLOS Pathogens: published February 4, 2025 | https://doi.org/10.1371/journal.ppat.1012813

Increased NFAT and NFκB signalling contribute to the hyperinflammatory phenotype in response to Aspergillus fumigatus in a mouse model of cystic fibrosis

Amelia Bercusson, Thomas J. Williams, Nicholas J. Simmonds, Eric WFW Alton, Uta Griesenbach, Anand Shah, Adilia Warris, Darius Armstrong-James

PLOS Pathogens: published February 4, 2025 | https://doi.org/10.1371/journal.ppat.1012784

CCR2 signaling regulates anti-chlamydia T cell immune responses in the airway

Shuaini Yang, Jinxi Yu, Xue Dong, Jiajia Zeng, Lu Tan, Hong Zhang, Ruoyuan Sun, Yuqing Tuo, Jing Yang, Chunxiao Wan, Hong Bai

PLOS Pathogens: published February 4, 2025 | https://doi.org/10.1371/journal.ppat.1012912

Enterovirus-like particles encapsidate RNA and exhibit decreased stability due to lack of maturation

Louis Kuijpers, Evdokia-Anastasia Giannopoulou, Yuzhen Feng, Wouter van den Braak, Abbas Freydoonian, Ramon Ramlal, Hugo Meiring, Belén Solano, Wouter H. Roos, Arjen J. Jakobi, Leo A. van der Pol, Nynke H. Dekker

PLOS Pathogens: published February 4, 2025 | https://doi.org/10.1371/journal.ppat.1012873

Antibiotics change the population growth rate heterogeneity and morphology of bacteria

Morten Kals, Emma Kals, Jurij Kotar, Allen Donald, Leonardo Mancini, Pietro Cicuta

PLOS Pathogens: published February 5, 2025 | https://doi.org/10.1371/journal.ppat.1012924

Activating PKC-ε induces HIV expression with improved tolerability

Alivelu M. Irrinki, Jasmine Kaur, Bally Randhawa, Ryan McFadden, Chelsea Snyder, Hoa Truong, Daniel Soohoo, Eric Hu, Helen Yu, Bernard P. Murray, Bing Lu, Dmytro Kornyeyev, Ishak Darryl Irwan, Lan Nguyen, Yu-San Yang, Jean-Philippe Belzile, Uli Schmitz, Todd C. Appleby, Brian Schultz, Jay Lalezari, Steven Deeks, Tomas Cihlar, Jeffrey P. Murry

PLOS Pathogens: published February 6, 2025 | https://doi.org/10.1371/journal.ppat.1012874

Host 3’ flap endonuclease Mus81 plays a critical role in trimming the terminal redundancy of hepatitis B virus relaxed circular DNA during covalently closed circular DNA formation

Hu Zhang, Quanxin Long, Yuanjie Liu, Alexander L. Marchetti, Cheng-Der Liu, Ning Sun, Haitao Guo

PLOS Pathogens: published February 6, 2025 | https://doi.org/10.1371/journal.ppat.1012918

Chromosomal genome assembly resolves drug resistance loci in the parasitic nematode Teladorsagia circumcincta

Jennifer McIntyre, Alison Morrison, Kirsty Maitland, Duncan Berger, Daniel R. G. Price, Sam Dougan, Dionysis Grigoriadis, Alan Tracey, Nancy Holroyd, Katie Bull, Hannah Rose Vineer, Mike J. Glover, Eric R. Morgan, Alasdair J. Nisbet, Tom N. McNeilly, Yvonne Bartley, Neil Sargison, Dave Bartley, Matt Berriman, James A. Cotton, Eileen Devaney, Roz Laing, Stephen R. Doyle

PLOS Pathogens: published February 6, 2025 | https://doi.org/10.1371/journal.ppat.1012820

Wnt/β-catenin signalling underpins juvenile Fasciola hepatica growth and development

Rebecca Armstrong, Nikki J. Marks, Timothy G. Geary, John Harrington, Paul M. Selzer, Aaron G. Maule

PLOS Pathogens: published February 7, 2025 | https://doi.org/10.1371/journal.ppat.1012562

E6AP is essential for the proliferation of HPV-positive cancer cells by preventing senescence

Alicia Avenhaus, Milica Velimirović, Julia Bulkescher, Martin Scheffner, Felix Hoppe-Seyler, Karin Hoppe-Seyler

PLOS Pathogens: published February 7, 2025 | https://doi.org/10.1371/journal.ppat.1012914

Alternative lipid synthesis in response to phosphate limitation promotes antibiotic tolerance in Gram-negative ESKAPE pathogens

Roberto Jhonatan Olea-Ozuna, Melanie J. Campbell, Samantha Y. Quintanilla, Sinjini Nandy, Jennifer S. Brodbelt, Joseph M. Boll

PLOS Pathogens: published February 7, 2025 | https://doi.org/10.1371/journal.ppat.1012933

Asymptomatic neonatal herpes simplex virus infection in mice leads to persistent CNS infection and long-term cognitive impairment

Abigail J. Dutton, Evelyn M. Turnbaugh, Chaya D. Patel, Callaghan R. Garland, Sean A. Taylor, Roberto Alers-Velazquez, David M. Knipe, Katherine M. Nautiyal, David A. Leib

PLOS Pathogens: published February 7, 2025 | https://doi.org/10.1371/journal.ppat.1012935

Repression of varicella zoster virus gene expression during quiescent infection in the absence of detectable histone deposition

Jiayi Wang, Nadine Brückner, Simon Weissmann, Thomas Günther, Shuyong Zhu, Carolin Vogt, Guorong Sun, Rongrong Guo, Renzo Bruno, Birgit Ritter, Lars Steinbrück, Benedikt B. Kaufer, Daniel P. Depledge, Adam Grundhoff, Abel Viejo-Borbolla

PLOS Pathogens: published February 10, 2025 | https://doi.org/10.1371/journal.ppat.1012367

Discovery of bimodal hepatitis B virus ribonuclease H and capsid assembly inhibitors

Daniel P. Bradley, Caleb J. Valkner, Qilan Li, Makafui Gasonoo, Marvin J. Meyers, Georgia-Myrto Prifti, Dimitrios Moianos, Grigoris Zoidis, Adam Zlotnick, John E. Tavis

PLOS Pathogens: published February 10, 2025 | https://doi.org/10.1371/journal.ppat.1012920

A guanidine-based coronavirus replication inhibitor which targets the nsp15 endoribonuclease and selects for interferon-susceptible mutant viruses

Benjamin Van Loy, Eugènia Pujol, Kenichi Kamata, Xiao Yin Lee, Nikolai Bakirtzoglou, Ria Van Berwaer, Julie Vandeput, Cato Mestdagh, Leentje Persoons, Brent De Wijngaert, Quinten Goovaerts, Sam Noppen, Maarten Jacquemyn, Kourosh Ahmadzadeh, Eline Bernaerts, Juan Martín-López, Celia Escriche, Bert Vanmechelen, Besir Krasniqi, Abhimanyu K. Singh, Dirk Daelemans, Piet Maes, Patrick Matthys, Wim Dehaen, Jef Rozenski, Kalyan Das, Arnout Voet, Santiago Vázquez, Lieve Naesens, Annelies Stevaert

PLOS Pathogens: published February 11, 2025 | https://doi.org/10.1371/journal.ppat.1012571

HBV polymerase recruits the phosphatase PP1 to dephosphorylate HBc-Ser170 to complete encapsidation

Chi-Ling Hsieh, Li-Yang Chang, Pei-Jer Chen, Shiou-Hwei Yeh

PLOS Pathogens: published February 11, 2025 | https://doi.org/10.1371/journal.ppat.1012905

Order among chaos: High throughput MYCroplanters can distinguish interacting drivers of host infection in a highly stochastic system

Melissa Y. Chen, Leah M. Fulton, Ivie Huang, Aileen Liman, Sarzana S. Hossain, Corri D. Hamilton, Siyu Song, Quentin Geissmann, Kayla C. King, Cara H. Haney

PLOS Pathogens: published February 11, 2025 | https://doi.org/10.1371/journal.ppat.1012894

Intracellular Pseudomonas aeruginosa persist and evade antibiotic treatment in a wound infection model

Stéphane Pont, Flore Nilly, Laurence Berry, Anne Bonhoure, Morgan A. Alford, Mélissande Louis, Pauline Nogaret, Manjeet Bains, Olivier Lesouhaitier, Robert E. W. Hancock, Patrick Plésiat, Anne-Béatrice Blanc-Potard

PLOS Pathogens: published February 13, 2025 | https://doi.org/10.1371/journal.ppat.1012922

Inhibition of the Integrated stress response by Epstein-Barr virus oncoprotein LMP1 attenuates epithelial cell differentiation and lytic viral reactivation

Deo R. Singh, Yitao Zhang, Sophia J. White, Scott E. Nelson, Stuart A. Fogarty, Abigail S. Pawelski, Alisha S. Kansra, Shannon C. Kenney

PLOS Pathogens: published February 14, 2025 | https://doi.org/10.1371/journal.ppat.1012934

Establishment of reverse genetics systems for Colorado tick fever virus

Shohei Minami, Ryotaro Nouda, Katsuhisa Hirai, Zelin Chen, Tomohiro Kotaki, Yuta Kanai, Takeshi Kobayashi

PLOS Pathogens: published February 14, 2025 | https://doi.org/10.1371/journal.ppat.1012921

Exploring the activity of the putative Δ6-desaturase and its role in bloodstream form life-cycle transitions in Trypanosoma brucei

Michela Cerone, Terry K. Smith

PLOS Pathogens: published February 18, 2025 | https://doi.org/10.1371/journal.ppat.1012691

Structures of two lyssavirus glycoproteins trapped in pre- and post-fusion states and the implications on the spatial-temporal conformational transition along with pH-decrease

Fanli Yang, Sheng Lin, Xin Yuan, Siqi Shu, Yueru Yu, Jing Yang, Fei Ye, Zimin Chen, Bin He, Jian Li, Qi Zhao, Haoyu Ye, Yu Cao, Guangwen Lu

PLOS Pathogens: published February 19, 2025 | https://doi.org/10.1371/journal.ppat.1012923

Structure-based design of an immunogenic, conformationally stabilized FimH antigen for a urinary tract infection vaccine

Natalie C. Silmon de Monerri, Ye Che, Joshua A. Lees, Jayasankar Jasti, Huixian Wu, Matthew C. Griffor, Srinivas Kodali, Julio Cesar Hawkins, Jacqueline Lypowy, Christopher Ponce, Kieran Curley, Alexandre Esadze, Juan Carcamo, Thomas McLellan, David Keeney, Arthur Illenberger, Yury V. Matsuka, Suman Shanker, Laurent Chorro, Alexey V. Gribenko, Seungil Han, Annaliesa S. Anderson, Robert G. K. Donald

PLOS Pathogens: published February 19, 2025 | https://doi.org/10.1371/journal.ppat.1012325

Isolation of a novel human prion strain from a PRNP codon 129 heterozygous vCJD patient

Fuquan Zhang, Susan Joiner, Jacqueline M. Linehan, Florin Pintilii, Tamsin Nazari, Fabio Argentina, Connor Preston, Maged Taema, Thomas J. Cunningham, Emmanuel A. Asante, Tzehow Mok, Simon Mead, Sebastian Brandner, John Collinge, Jonathan D.F. Wadsworth

PLOS Pathogens: published February 20, 2025 | https://doi.org/10.1371/journal.ppat.1012904

Interaction of the endogenous antibody response with activating FcγRs enhance control of Mayaro virus through monocytes

Megan M. Dunagan, Nathânia Dábilla, Colton McNinch, Jason M. Brenchley, Patrick T. Dolan, Julie M. Fox

PLOS Pathogens: published February 24, 2025 | https://doi.org/10.1371/journal.ppat.1012944

A ribosome-interacting jumbophage protein associates with the phage nucleus to facilitate efficient propagation

Wichanan Wannasrichan, Sucheewin Krobthong, Chase J. Morgan, Emily G. Armbruster, Milan Gerovac, Yodying Yingchutrakul, Patompon Wongtrakoongate, Jörg Vogel, Chanat Aonbangkhen, Poochit Nonejuie, Joe Pogliano, Vorrapon Chaikeeratisak

PLOS Pathogens: published February 24, 2025 | https://doi.org/10.1371/journal.ppat.1012936

Citrus tristeza virus p20 suppresses antiviral RNA silencing by co-opting autophagy-related protein 8 to mediate the autophagic degradation of SGS3

Yongle Zhang, Zuokun Yang, Zhe Zhang, Guoping Wang, Xiang-Dong Li, Ni Hong

PLOS Pathogens: published February 24, 2025 | https://doi.org/10.1371/journal.ppat.1012960

Liver stage P. falciparum antigens highly targeted by CD4+ T cells in malaria-exposed Ugandan children

Gonzalo R. Acevedo, Sophie S. Samiee, Mikias Ilala, Justine Levan, Meagan E. Olive, Riana D. Hunter, Mary Prahl, Raja Rajalingam, John Rek, Grant Dorsey, Margaret E. Feeney

PLOS Pathogens: published February 24, 2025 | https://doi.org/10.1371/journal.ppat.1012943

Short Reports

Argonaute 2 targets viral transcripts but not genomes of RNA viruses during antiviral RNA interference in Drosophila

Emanuele G. Silva, Isaque J. S. de Faria, Álvaro G. A. Ferreira, Thiago Henrique L. Jiran, Carlos F. Estevez-Castro, Juliana N. Armache, Siad C. G. Amadou, Yann Verdier, Joëlle Vinh, Karim Majzoub, Carine Meignin, Gabrielle Haas, Franck Martin, Jean-Luc Imler, João T. Marques

PLOS Pathogens: published February 3, 2025 | https://doi.org/10.1371/journal.ppat.1012184