Figures
There are errors in Fig 3. The β actin loading control blot in Fig 3C was in the wrong orientation in the submission. Please see the correct Fig 3 here.
A. Nitric oxide levels in conditioned media in response to SIRT2 inhibition at indicated time post infection. (UI- uninfected DCs, UI AK7- Uninfected DCs treated with 10 μM AK7, STM- Salmonella Typhimurium infected DCs, STM AK7- - Salmonella Typhimurium infected DCs treated with 10 μM AK7) (Data are presented as mean ± SEM of 5 independent experiments). B. qPCR analysis of NOS2 expression in DCs at indicated time post infection in response to SIRT2 inhibition. GAPDH was used as an internal control. (UI- uninfected DCs, UI AK7- Uninfected DCs treated with 10 μM AK7, STM- Salmonella Typhimurium infected DCs, STM AK7- Salmonella Typhimurium infected DCs treated with 10 μM AK7, LPS- 100 ng/ml LPS treated). (Data are presented as mean ± SEM of 3 independent experiments). C. Representative immunoblot of NOS2 in the presence and absence of SIRT2 inhibition at indicated time. (UI- uninfected DCs, UI AK7- Uninfected DCs treated with 10 μM AK7, STM- Salmonella Typhimurium infected DCs, STM AK7- Salmonella Typhimurium infected DCs treated with 10 μM AK7) D. Densitometry analysis of NOS2 level in the presence and absence of SIRT2 inhibition at indicated time. (UI- uninfected DCs, UI AK7- Uninfected DCs treated with 10 μM AK7, STM- Salmonella Typhimurium infected DCs, STM AK7- Salmonella Typhimurium infected DCs treated with 10 μM AK7) (Data are presented as mean ± SEM of 3 independent experiments). E. Percentage survival of intracellular Salmonella in DCs in gentamicin protection assay in the presence and absence of SIRT2 inhibitor in wild type and NOS2-/- DCs. (Mock- DMSO treated, AK7- 10 μM AK7 treated). (Data are presented as mean ± SEM of 4 independent experiments) F. 3[H] Thymidine incorporation assay to assess CD8+ T cells proliferation during Salmonella infection in wild type and NOS2-/- DCs in the presence and absence of SIRT2 inhibition. (Ova- Ovalbumin, DC- Dendritic cells, T cell- mixed lymphocyte, STM- Salmonella Typhimurium, ConA- Concanavalin A) (Data are presented as mean ± SEM of 3 independent experiments) G. IL-2 levels during CD8+T cells proliferation assay in response to Salmonella infection in wild type and NOS2-/- DCs. (Ova- Ovalbumin, DC- Dendritic cells, T cell- mixed lymphocyte, STM- Salmonella Typhimurium) (Data are presented as mean ± SEM of 2 independent experiments) (unpaired two tailed Student’s t- test, p- value, *** < 0.0001, **<0.001, *<0.01).
Reference
- 1. Gogoi M, Chandra K, Sarikhani M, Ramani R, Sundaresan NR, Chakravortty D (2018) Salmonella escapes adaptive immune response via SIRT2 mediated modulation of innate immune response in dendritic cells. PLoS Pathog 14(11): e1007437. https://doi.org/10.1371/journal.ppat.1007437 pmid:30452468
Citation: Gogoi M, Chandra K, Sarikhani M, Ramani R, Sundaresan NR, Chakravortty D (2020) Correction: Salmonella escapes adaptive immune response via SIRT2 mediated modulation of innate immune response in dendritic cells. PLoS Pathog 16(2): e1008345. https://doi.org/10.1371/journal.ppat.1008345
Published: February 5, 2020
Copyright: © 2020 Gogoi et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.