Figures

The instructions on this page pertain to figures included in the main article.

Before we can formally accept your submission, your figures must meet the requirements on this page.
The more closely your figures adhere to these specifications at full submission, the fewer times you will need to revise your manuscript to meet the requirements. Your figures will therefore be less likely to slow down publication of your accepted manuscript.

Figures as Supporting Information

Supporting information is auxiliary to the main content of the article. Supporting information figures are held to the requirements of all supporting information files. They have fewer requirements than figures that are included in the main article, and they need to be uploaded separately.

For full instructions, follow the supporting information guidelines.

Figure Preparation Checklist

Read our figure policies on depictions of humans, licenses and copyright, and image manipulation.
Read the figure file requirements for the full list of technical specifications, and ensure your figures comply.
Read how to format and submit your figures and captions for peer review.
Use PACE before submitting to check your figures and convert to our accepted formats.

Figure File Requirements

The list below is an abbreviated summary of the figure specifications. Read the full details of the requirements in the corresponding sections on this page.

File Format	TIFF or EPS
Dimensions	Width: 789 – 2250 pixels (at 300 dpi). Height maximum: 2625 pixels (at 300 dpi).
Resolution	300 – 600 dpi
File Size	<10 MB
Text within Figures	Arial, Times, or Symbol font only in 8-12 point
Figure Files	Fig1.tif, Fig2.eps, and so on. Match file name to caption label and citation.
Captions	In the manuscript, not in the figure file.

File Format	TIFF or EPS only. Should I choose TIFF or EPS? TIFF tends to be easier to work with than EPS. EPS files often have missing/corrupted fonts, oversized masks, stray points, and boxes, which can result in errors and poor output.
Dimensions		Centimeters	Inches	Pixels at 300 dpi
	Minimum width	6.68	2.63	789
	Maximum width	19.05	7.5	2250
	Height maximum At the height maximum, the figure occupies the whole page and excludes the caption	22.23	8.75	2625
	“Dimensions” refers to the dimensions of the entire figure, excluding any white space. The closer figures match these dimensions, the closer they will meet expectations on publication. TIPS To align your figure with the text column of the PDF version of the article, make it no wider than 5.2 inches (13.2 cm). Ensure that individual images meet the pixel dimensions indicated above when preparing and saving figures with slideshow software (like Powerpoint, OpenOffice, or Keynote). Make sure source images are 2,250 pixels wide if you want figures at full page width of 19.05cm. When adjusting the figure dimensions, be sure the Chain/Lock symbol is closed. This will maintain the width and height ratio of your figure. Go to Image → Properties (or Image → Information) to view image dimensions.
Resolution	Submit figures at the desired dimensions with a resolution no greater than 300-600 dpi. The quality of a low-resolution figure cannot be improved by simply increasing the resolution in graphics software. To improve its resolution, re-create the figure from the beginning. Resolution below 300 results in blurred, jagged or pixelated published figures. Resolution above 600 dpi may lead to resizing of the published figure. The quality of your figures is only as good as the lowest-resolution element present. If you created a 72 dpi line graph and placed it in a 300 dpi TIFF, the graph will look blurred, jagged, or pixilated.
File Size	Submit files at a size of 10 MB or less. Use the PACE tool for help resizing. If you elect not to use PACE, follow the instructions below to manually resize TIFF or EPS files. Reducing TIFF file size Save with LZW compression. Set resolution between 300-600 dpi. Flatten. A flattened TIFF has a single layer called “background” and has a smaller file size than a TIFF with “Layer 1”. Reducing EPS file size Source images created in EPS format need to be compressed (in your compression format of choice) and should be no larger than full page size. If your EPS files are still too large, convert them to PDF and then export to a compressed TIFF.
Text within Figures	Use only Arial, Times, or Symbol font in 8-12 point. Do not include author names, article title, or figure number/title/caption within figure files. That information will go into your figure caption in the manuscript. Read more about submitting captions. Text within EPS figures Embed fonts, or convert them to outlines to prevent missing or improperly rendered text. In those files that are created in software like Matlab, open Illustrator or Inkscape and convert text to outlines. You will not be able to change your text after it has been converted to outlines. Instructions for Illustrator 1. Select all (Ctrl + A) 2. Shift + Control + O on PC (Shift + Command + O on Mac) Instructions for Inkscape 1. Select all (Ctrl + A) 2. Shift + Ctrl + C
Multi-panel Figures	Place all panels from a multi-part figure into a single page and single file: Combine multiple panels into one page, or break them apart into separate figures. Renumber all figures and in-text citations accordingly. To create a multi-panel figure from individual files, use a presentation program such as OpenOffice Impress, Microsoft PowerPoint, or Keynote for Mac. Then convert to TIFF. To set up the page, use the values listed in Dimensions. Use an Insert tool to place figures. Do not drag/drop or copy/paste images into the file, because this results in a 72 dpi image. If your figures have numerous pictures, charts, or small text, they will render best at a resolution of 600 dpi.
Color Mode	RGB (8 bit/channel) or grayscale only.
White Space	A 2-point white space border around each figure is recommended to prevent inadvertent cropping of content at layout. Crop out excess white space around image content.
Orientation	Rotate and submit the figure in the orientation that you wish it to publish. Figures will be inserted into the typeset article in the orientation in which they are supplied. For example, if a vertical image is submitted in a horizontal orientation, it will be set horizontally in the article.

Additional Requirements for TIFF

Layers	Flattened, with no layers. Figures with a single layer named “layer 1” or “layer 0” are in fact layered.
Alpha Channels	No alpha channels.
Compression	LZW compression is required. To apply: In GIMP, use “Export” instead of “Save As”. Select TIFF as the format, and then select LZW compression. In Photoshop, select “LZW compression” and “Discard Layers, and Save a Copy.”
Pages	We cannot publish TIFF figures that span multiple pages. To combine multiple figures onto a single page, see Multi-panel Figures.

How to Submit Figures and Captions

Your figures contain three elements: figure files, captions, and in-text citations.

When naming your figures, match the figure file name and the caption label with the corresponding in-text citations in the manuscript. Example: a figure file named “Fig1.tif” should match the citation “Fig 1” and the figure label “Fig 1.” in the caption.

Figure files	Initial & full submissions You can choose to format your submission as either: A single PDF containing the manuscript, figures, and supporting information, or A PDF or Word document manuscript, with figures and supporting information uploaded individually Revised submissions Name files in numeric order with the format: Fig1.tif, Fig2.eps, and so on. Upload each figure as an individual file that is separate from the manuscript. If at any point you change the numbering order of your figures, be sure to update all figure citations, captions, and file names accordingly.
Captions	Place each caption in the manuscript text in read order, immediately following the paragraph where the figure is first cited. Do not include captions as part of the figure files or submit them in a separate document. Format your figure captions. There are two required elements: figure label and figure title. Legends are optional. Label. Name figure labels using Arabic numerals, and abbreviate the word “Figure” to “Fig” (e.g., Fig 1, Fig 2, Fig 3, etc.). Title. The title should be concise and descriptive. Restrict it to 15 words or less. Legend. Place the legend directly after the title of the figure to which it belongs. Place any figure credits in the last sentence of the legend. Caption credit: Means JC, Venkatesan A, Gerdes B, Fan J-Y, Bjes ES, Price JL (2015) Drosophila Spaghetti and Doubletime Link the Circadian Clock and Light to Caspases, Apoptosis and Tauopathy. PLoS Genet 11(5): e1005171. https://doi.org/10.1371/journal.pgen.1005171 Figure legends tips Be succinct: Avoid lengthy descriptions of methods. Describe the key messages of a figure: provide a description of the figure that will allow readers to understand it without referring to the text. Describe each part of a multipart figure with with a lettered panel label: for example, (A) or (a). Define all non-standard symbols and abbreviations.
In-text citations	Cite figures with the format: Fig 1A, Fig 1B, Fig 2, Fig 3, etc. Cite figures in ascending numeric order upon first appearance in the manuscript file. This includes citations to text boxes and tables. In the published article, figures are inserted according to the placement of their first citation and caption in the article. Lettered subparts of whole figures may be cited in any order in the text if the first mention of each whole figure is in numerical order. For example, you can cite any subpart of Fig 3 in any order (e.g., Fig 3C before Fig 3A), as long as Figs 1 and 2 have already been cited.

Figure files

Initial & full submissions

You can choose to format your submission as either:

A single PDF containing the manuscript, figures, and supporting information, or
A PDF or Word document manuscript, with figures and supporting information uploaded individually

Revised submissions

Name files in numeric order with the format: Fig1.tif, Fig2.eps, and so on.
Upload each figure as an individual file that is separate from the manuscript.

If at any point you change the numbering order of your figures, be sure to update all figure citations, captions, and file names accordingly.

Captions

Place each caption in the manuscript text in read order, immediately following the paragraph where the figure is first cited. Do not include captions as part of the figure files or submit them in a separate document.
Format your figure captions. There are two required elements: figure label and figure title. Legends are optional.

Label. Name figure labels using Arabic numerals, and abbreviate the word “Figure” to “Fig” (e.g., Fig 1, Fig 2, Fig 3, etc.).

Title. The title should be concise and descriptive. Restrict it to 15 words or less.

Legend. Place the legend directly after the title of the figure to which it belongs. Place any figure credits in the last sentence of the legend.

Caption credit: Means JC, Venkatesan A, Gerdes B, Fan J-Y, Bjes ES, Price JL (2015) Drosophila Spaghetti and Doubletime Link the Circadian Clock and Light to Caspases, Apoptosis and Tauopathy. PLoS Genet 11(5): e1005171. https://doi.org/10.1371/journal.pgen.1005171

Figure legends tips

Be succinct: Avoid lengthy descriptions of methods.
Describe the key messages of a figure: provide a description of the figure that will allow readers to understand it without referring to the text.
Describe each part of a multipart figure with with a lettered panel label: for example, (A) or (a).
Define all non-standard symbols and abbreviations.

In-text citations

Cite figures with the format: Fig 1A, Fig 1B, Fig 2, Fig 3, etc.
Cite figures in ascending numeric order upon first appearance in the manuscript file. This includes citations to text boxes and tables. In the published article, figures are inserted according to the placement of their first citation and caption in the article.
Lettered subparts of whole figures may be cited in any order in the text if the first mention of each whole figure is in numerical order. For example, you can cite any subpart of Fig 3 in any order (e.g., Fig 3C before Fig 3A), as long as Figs 1 and 2 have already been cited.

Tools for Figure Preparation

Although our staff can offer guidance and advice, PLOS does not provide graphics services. Preparation and final quality of figures is the responsibility of the author.

Automated figure assessment

PLOS provides a free, simple-to-use tool called PACE to help you review and prepare your figures for submission and achieve high publication quality figure presentation.

PACE will assess whether your figures meet our technical requirements by comparing each figure against our figure specifications (see Figure File Requirements). The tool can also convert figures to TIFF format, resize, and rename figures to our file naming conventions. In cases where PACE cannot process your figure file, the tool will provide you with a report of what it could not fix. Please note that figures must be captured between 300 and 600 dpi in order for PACE to successfully process your figures.

NOTE: Supporting Information figures should not be processed through PACE.

Please review your figures after PACE’s assessment to ensure that they adhere to journal figure guidelines and that they are not blurry or difficult to read.

To use PACE, first register as a user.

Go to PACE now.

Software

If you prefer to assemble figures with vector graphics, we recommend that you use Adobe Illustrator or Inkscape (free). These figures must be exported to EPS format per our requirements.

If you prefer to use raster graphics, the following free programs can both manipulate and export TIFF files:

LaTeX

PLOS does not accept vector EPS figures generated in LaTeX. Submit TIFF or EPS figures created in standard software. Read about other software options.

Converting LaTeX-generated EPS figures to TIFF

Compile the LaTeX files into a PDF.
Open the PDF in Photoshop, GIMP, or another graphics program that enables TIFF.
Crop out the figure and export as TIFF.

Creating Source Images with Specialized Software

While we cannot offer assistance using the software in this section, the instructions below indicate the proper settings for creating specialized scientific images that meet our figure requirements.

SigmaPlot

Creating a PLOS compatible graph

First create your graph, and save it in SigmaPlot format.
From the Tools menu select Options. In the dialogue box that appears, click on the Page tab. Set the Units to Millimeters (mm) and make sure the Graph Objects → Resize with Graph option is not ticked. Click OK.
From the file menu select Page Setup. In the dialogue box that appears, click on the Margins tab. Set all the margins to 0.0 mm, then click Apply.
Now click on the Page Size tab. Set the Width to 190.5 mm (or 132.0 mm for text column width) and the Height to 222.3 mm. Click OK.
Set the font size of all text to 8 pt, and the width of all lines to 0.2 mm. (Consult the SigmaPlot Help files for more details, if needed.)
Resize your graph to fit within and make full use of the page width available.

Saving an image in PDF format

This is the preferred output format when using SigmaPlot. To make sure your image is saved in a compatible format, perform the following steps.

From the File menu in SigmaPlot, select Print. In the Print dialogue box that appears, select Adobe PDF as the printer. Click on Properties.
Change the Default Settings pull-down to Press Quality. Uncheck the View Adobe PDF results box if you don't want Acrobat to launch.
Click OK, then click OK. Pick where the PDF will be created, and click Save.
Lastly, open the PDF in one of the following three programs to save the file in TIFF format: PACE, Photoshop, or GIMP.

RStudio

Export figures from RStudio in EPS format. If you prefer to submit a TIFF file, use PACE to do the conversion and achieve a resolution of 300dpi. TIFF files exported directly from RStudio will only achieve a resolution of 72dpi.

Image format: EPS
Width: 789 pixels at minimum
Check “Maintain aspect ratio”
Submit in EPS format, or use PACE to convert to TIFF

The table below provides the export settings and instructions for authors that choose to create TIFF source images with the software indicated.

Software	Settings and Instructions
Matlab	Knowing the target size of your image in inches and dpi, first convert the number of rows and columns in the image. 4.86 inches by 9.19 inches (a 1.5 column figure at maximum height) at 300 dpi corresponds to 1458 pixels by 2757 pixels. Modify your image to be 1485 by 2757. (Resize it, crop it, compute it differently, etc.) Then save your 1458 by 2757 image as a TIFF file, specifying 300 as the resolution. imwrite(my_image, ‘figure_10.tif’, ‘Resolution’, 300); function write Fig 300 dpi (figNo, fileName) %make the background white Set(figNo,’color’,’w’; f=getframe(figNo); colormap(f.colormap) imwrite(f.cdata, fileName,’ Resolution’, 300;
Prism	Export your graph with the following settings: File format: TIFF Resolution: 300 Color Mode: RGB Size Make Width: 7.5 in Enable Compression
Stata	Stata does everything at screen resolution (72 dpi). If you want to have a panel that is half page width, export the image at 39.52 cm. For full page width, export at 79.25 cm.
ChemDraw	Export to SVG (Windows Version only). The SVG format, like a PDF or EPS, is a vector based graphic format. GIMP and Photoshop will treat it in the same way as they would a PDF. Use Photoshop or GIMP to edit your figure.
PyMol	To get a 300 dpi PNG file for a 4.92 inch by 9.25 inch image, use the following settings. Ray-traced: Ray 1458,2757 Pnghires_ray.pdb, dpi=300 OpenGL: Draw 1458,2757 Pnghires_ogl.pdb, dpi=300
SPSS	Export a PDF, then bring that PDF into Photoshop or GIMP.
GeneSpring	Export as Image. Unit: cm. Print Width: 19.05, Lock Aspect Ratio, Image Resolution (in dpi): 600.
Minitab	“Save Graph As” with the following settings: Save as Type: TIF Color: RGB Custom Resolution: 600

Maps

PLOS applies the Creative Commons Attribution (CC BY) license to all the works we publish. Read about our licenses and copyright policy for figures.

When creating a figure that includes a map, be sure to check the usage rights. Several sites provide maps that are compatible with CC BY licensing, including:

USGS: all USGS maps are in the public domain
SEDAC: many maps from SEDAC are available under an Open Access license
World of Maps: may include maps in the public domain
OpenStreetMap: OpenStreetMap map tiles are free to use as long as they are accompanied by the following attribution statement: “Base map and data from OpenStreetMap and OpenStreetMap Foundation”. Maps created using OpenStreetMap data must be accompanied by the following attribution statement: "Contains information from OpenStreetMap and OpenStreetMap Foundation, which is made available under the Open Database License.”

A map that is free to download may be restricted in other ways. Do not use Google, Mapquest, or other copyrighted maps.

PLOS neither endorses nor takes responsibility for the maps or geodata from the above sites.

Policies

All figures must comply with PLOS policies covering depictions of humans, and licenses and copyright.

Depictions of humans

Authors submitting manuscripts that include identifying or potentially identifying information must comply with our requirements for informed consent.

Identifying information includes, but is not limited to:

photographs
radiographs
pedigrees (family trees)
geospatial maps that can identify a specific location such as a house.

Read our guidelines for documenting informed consent. If you require further information, please contact the journal before submitting.

Licenses and copyright

Figures, tables, and images are published under the Creative Commons Attribution (CC BY) license.

Do not submit any figures, photos, tables, or other works that have been previously copyrighted or that contain proprietary data unless you have and can supply written permission from the copyright holder to use that content.

This includes:

maps and satellite images
slogans and logos
social media content.

Read the full licenses and copyright policy.

Image manipulation

Image files should not be manipulated or adjusted in any way that could lead to misinterpretation of the information present in the original image. We routinely check figures for all accepted articles. Please be aware that we may also require you to supply us with your original photographic images in the original file format and at the resolution at which they were first created.

Avoiding image manipulation

We realize that the extent to which figures can be changed as part of normal preparation can pose a dilemma. Please refer to the general guidance below on aspects to consider when preparing your figures.

For further information, image examples, and more detailed guidance we advise reading What’s in a picture? The temptation of image manipulation (reprinted in the Journal of Cell Biology, 2004;166(1):11-15).

We are grateful to staff at the Journal of Cell Biology (Rockefeller University Press) for their help in establishing these guidelines and procedures.

Common issues to avoid

The following examples are all considered to be unacceptable image manipulation:

The introduction, enhancement, movement, or removal of specific feature(s) within an image

EXAMPLES: See Figures 1 and 5 of What’s in a picture? The temptation of image manipulation.

Unmarked grouping of images that should otherwise have been presented separately (for example, from different parts of the same gel, or from different gels, fields, or exposures)

EXAMPLE: See Figure 6 of What’s in a picture? The temptation of image manipulation.

Adjustments of brightness, contrast, or color balance that obscure, eliminate, or misrepresent any information

EXAMPLE: See Figure 3 of What’s in a picture? The temptation of image manipulation.

Creating high-quality images

Poor-quality images may raise figure manipulation queries and/or result in requests to remake figures. Follow these tips for creating high-quality images:

Ensure that your images have a resolution of at least 300 pixels per inch (ppi) and appear sharp, not pixelated. Poor-quality images cannot be checked.
Be careful not to inadvertently reduce the resolution when creating a file in graphics editing software

When opening your image file in graphics editing software, you have the option of setting the size and resolution of the image. Do not set the total number of pixels to be greater than that in the original image (e.g., from the digital camera attached to your microscope) while keeping the image size the same; otherwise, the computer must create data for you that were not present in the original, and the resulting image is a misrepresentation of the original data. The resolution (ppi) can be increased only if the image size is reduced proportionately.

Spliced gels need a thin dividing line added to indicate the alteration. It is fine to remove a complete lane and splice the remaining lanes together, but the alteration must be indicated by a thin white or black line between the juxtaposed pieces.
Images must contain the background “noise” they originally contained. The background should not appear as one uniform color. Do not “clean up” the background of images with rubberstamp or “wipe” tools to improve the aesthetic appearance, or over-adjust the brightness or contrast so that the background is removed.

EXAMPLE: See Figure 4 of What’s in a picture? The temptation of image manipulation.

Contact

If you still have questions about how to prepare your figures, please contact figures@plos.org for assistance.

Figures

Figure Preparation Checklist

Figure File Requirements

File Format

Dimensions

Resolution

File Size

Text within Figures

Multi-panel Figures

Color Mode

White Space

Orientation

Additional Requirements for TIFF

Layers

Alpha Channels

Compression

Pages