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Table of Contents

June 2021

A low-income urban community located in the northern side of Dhaka, Bangladesh. Sultana, et al. (2021)

Image Credit: Khaled Saifullah and Humayun Kabir

Editorial

PLOS Neglected Tropical Diseases broadens its coverage of envenomings caused by animal bites and stings

José María Gutiérrez, Jean Philippe Chippaux, Geoffrey K. Isbister

Viewpoints

Noma, a neglected disease: A viewpoint article

Elise Farley, Cono Ariti, Mohana Amirtharajah, Charity Kamu, Bukola Oluyide, Muhammad Shoaib, Shafiu Isah, Adeniyi Semiyu Adetunji, Fatima Saleh, Chikwe Ihekweazu, Monique Pereboom, Mark Sherlock

Appraisal of antivenom production in public laboratories in Latin America during the first semester of 2020: The impact of COVID-19

José María Gutiérrez, Larissa Zanette, Marco Antonio Natal Vigilato, Julio Cesar Augusto Pompei, Diogo Martins, Hui Wen Fan, the Latin American Network of Public Antivenom Manufacturing Laboratories (RELAPA)

The 2021 Ebola virus outbreak in Guinea: Mistrust and the shortcomings of outbreak surveillance

Manuel Raab, Emmanuelle Roth, Vinh-Kim Nguyen, Guenter Froeschl

Reviews

Oral manifestations in chikungunya patients: A systematic review

Daniela Brostolin da Costa, Alessandro Diogo De-Carli, Livia Fernandes Probst, Antonio José Grande, Ana Tereza Gomes Guerrero

Diagnosing point-of-care diagnostics for neglected tropical diseases

Mitasha Bharadwaj, Michel Bengtson, Mirte Golverdingen, Loulotte Waling, Cees Dekker

Past and present of cystic echinococcosis in Bolivia

Viterman Ali, Eddy Martinez, Pamela Duran, Erick Villena, Peter Deplazes, Cristian A. Alvarez Rojas

Schistosomal appendicitis: Case series and systematic literature review

Mateus Zacarias, Damiano Pizzol, Helder de Miranda, Anna Claudia Colangelo, Nicola Veronese, Lee Smith

Research Articles

Comparative palatability of orally disintegrating tablets (ODTs) of Praziquantel (L-PZQ and Rac-PZQ) versus current PZQ tablet in African children: A randomized, single-blind, crossover study

Muhidin K. Mahende, Eric Huber, Elly Kourany-Lefoll, Ali Ali, Brooke Hayward, Deon Bezuidenhout, Wilhelmina Bagchus, Abdunoor M. Kabanywanyi

Precise localization and dynamic distribution of Japanese encephalitis virus in the rain nuclei of infected mice

Wei Han, Mingxing Gao, Changqing Xie, Jinhua Zhang, Zikai Zhao, Xueying Hu, Wanpo Zhang, Xiaoli Liu, Shengbo Cao, Guofu Cheng, Changqin Gu

Automated phenotyping of mosquito larvae enables high-throughput screening for novel larvicides and offers potential for smartphone-based detection of larval insecticide resistance

Steven D. Buckingham, Frederick A. Partridge, Beth C. Poulton, Benjamin S. Miller, Rachel A. McKendry, Gareth J. Lycett, David B. Sattelle

Effectiveness of a multifaceted prevention programme for melioidosis in diabetics (PREMEL): A stepped-wedge cluster-randomised controlled trial

Pornpan Suntornsut, Prapit Teparrukkul, Gumphol Wongsuvan, Wipada Chaowagul, Susan Michie, Nicholas P. J. Day, Direk Limmathurotsakul

Covid-19 in end-stage renal disease patients with renal replacement therapies: A systematic review and meta-analysis

Tanawin Nopsopon, Jathurong Kittrakulrat, Kullaya Takkavatakarn, Thanee Eiamsitrakoon, Talerngsak Kanjanabuch, Krit Pongpirul

Diversity of trypanosomes in humans and cattle in the HAT foci Mandoul and Maro, Southern Chad—A matter of concern for zoonotic potential?

Mahamat Alhadj Moussa Ibrahim, Judith Sophie Weber, Sen Claudine Henriette Ngomtcho, Djoukzoumka Signaboubo, Petra Berger, Hassane Mahamat Hassane, Sørge Kelm

Mark-release-recapture of male Aedes aegypti (Diptera: Culicidae): Use of rhodamine B to estimate movement, mating and population parameters in preparation for an incompatible male program

Brendan J. Trewin, Daniel E. Pagendam, Brian J. Johnson, Chris Paton, Nigel Snoad, Scott A. Ritchie, Kyran M. Staunton, Bradley J. White, Sara Mitchell, Nigel W. Beebe

Exploratory analysis of the effect of helminth infection on the immunogenicity and efficacy of the asexual blood-stage malaria vaccine candidate GMZ2

Odilon Nouatin, Juliana Boex Mengue, Jean Claude Dejon-Agobé, Rolf Fendel, Javier Ibáñez, Ulysse Ateba Ngoa, Jean Ronald Edoa, Bayodé Roméo Adégbité, Yabo Josiane Honkpéhédji, Jeannot Fréjus Zinsou, Aurore Bouyoukou Hounkpatin, Kabirou Moutairou, Andreas Homoet, Meral Esen, Andrea Kreidenweiss, Stephen L. Hoffman, Michael Theisen, Adrian J. F. Luty, Bertrand Lell, Selidji Todagbe Agnandji, Ghyslain Mombo-Ngoma, Michael Ramharter, Peter Kremsner, Benjamin Mordmüller, Ayôla Akim Adegnika

The human immune response to saliva of Phlebotomus alexandri, the vector of visceral leishmaniasis in Iraq, and its relationship to sand fly exposure and infection

Ines Lakhal-Naouar, Rami Mukbel, Robert F. DeFraites, Rupal M. Mody, Lina N. Massoud, Dutchabong Shaw, Edgie M. Co, Jeffrey E. Sherwood, Shaden Kamhawi, Naomi E. Aronson

A facility and community-based assessment of scabies in rural Malawi

Cristina Galván-Casas, Oriol Mitjá, Sara Esteban, Jacob Kafulafula, Texon Phiri, Íñigo Navarro-Fernández, Concepción Román-Curto, Hassani Mtenje, Gerald Thauzeni, Elizabeth Harawa, Stephano Kaluzi, Mphatso Diere, Mary Mkandawire, Shaibu Malizani, Alex Chifundo, Marta Utrera-Busquets, Mónica Roncero-Riesco, Sara López Martín-Prieto, Iosune Vilanova-Urdániz, Gisela H. Petiti, María Victoria de Gálvez Aranda, Nuria No Pérez, María Rueda Gómez-Calcerrada, Pilar Iranzo, Pilar Escalonilla García-Patos, Magdalena de Troya-Martín, Javier Romero Gomez, Esther Cardeñoso-Alvarez, Sofia Lucas Truyols, Libe Aspe Unanue, Cristina Bajo del Pozo, Alicia Comunión Artieda, Maria Isabel Martínez González, Omar F. López-López, Esther Moreno-Artero, Xavier Cubiró, Iago Meilán-Sánchez, Alejandra Tomás-Velázquez, Cristina López-Sánchez, Eva M. Sánchez-Martínez, Harrison A. Edwards, Maria Herrera Morueco, Julia Zehe Rubiera, Laura Salguero Caldera, Urbano Blanes-Moreno, Maria Uribarren-Movilla, Michael Marks

High risk of early sub-therapeutic penicillin concentrations after intramuscular benzathine penicillin G injections in Ethiopian children and adults with rheumatic heart disease

Ezra B. Ketema, Nigus Z. Gishen, Abraha Hailu, Abadi Leul, Abera Hadgu, Kiflom Hagos, Samual Berhane, Temesgen Tsega, Madhu Page-Sharp, Timothy ME Davis, Brioni Moore, Kevin T. Batty, Jonathan Carapetis, Sam Salman, Laurens Manning

Use of vector control to protect people from sleeping sickness in the focus of Bonon (Côte d’Ivoire)

Dramane Kaba, Vincent Djohan, Djakaridja Berté, Bi Tra Dieudonné TA, Richard Selby, Koffi Alain De Marie Kouadio, Bamoro Coulibaly, Gabehonron Traoré, Jean-Baptiste Rayaisse, Pierre Fauret, Vincent Jamonneau, Kouakou Lingue, Phillipe Solano, Steve J. Torr, Fabrice Courtin

Feasibility of a dried blood spot strategy for serological screening and surveillance to monitor elimination of Human African Trypanosomiasis in the Democratic Republic of the Congo

Raquel Inocencio da Luz, Delphin Mavinga Phanzu, Oscar N’lemvo Kiabanzawoko, Eric Miaka, Paul Verlé, Anja De Weggheleire, Philippe Büscher, Epco Hasker, Marleen Boelaert

Correlation between renal distribution of leptospires during the acute phase and chronic renal dysfunction in a hamster model of infection with Leptospira interrogans

Tsukasa Maruoka, Yasuhiko Nikaido, Satoshi Miyahara, Eisuke Katafuchi, Yoshinori Inamasu, Midori Ogawa, Kazumasa Fukuda, Toshiyuki Nakayama, Takafumi Horishita, Mitsumasa Saito

Cases and distribution of visceral leishmaniasis in western São Paulo: A neglected disease in this region of Brazil

Regiane Soares Santana, Karina Briguenti Souza, Fernanda Lussari, Elivelton Silva Fonseca, Cristiane Oliveira Andrade, Marcia Mitiko Kaihara Meidas, Lourdes Aparecida Zampieri D’Andrea, Francisco Assis Silva, Edilson Ferreira Flores, Ivete Rocha Anjolete, Luiz Euribel Prestes-Carneiro

Evidence of Zika virus circulation in asymptomatic pregnant women in Northeast, Brazil

Rebeca Costa Castelo Branco, Patrícia Brasil, Josélio Maria Galvão Araújo, Flávia Oliveira Cardoso, Zulmira Silva Batista, Valéria Maria Souza Leitão, Marcos Antonio Custódio Neto da Silva, Lailson Oliveira de Castro, Joanna Gardel Valverde, Selma Maria Bezerra Jeronimo, Josélia Alencar Lima, Raimunda Ribeiro da Silva, Maria do Carmo Lacerda Barbosa, Luciane Maria Oliveira Brito, Marcelo Antônio Pascoal Xavier, Maria do Desterro Soares Brandão Nascimento

Technical viability of the YF MAC-HD ELISA kit for use in yellow fever-endemic regions

Christin H. Goodman, Maurice Demanou, Mick Mulders, Jairo Mendez-Rico, Alison Jane Basile

The epidemiology of Mayaro virus in the Americas: A systematic review and key parameter estimates for outbreak modelling

Edgar-Yaset Caicedo, Kelly Charniga, Amanecer Rueda, Ilaria Dorigatti, Yardany Mendez, Arran Hamlet, Jean-Paul Carrera, Zulma M. Cucunubá

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A novel substrate for arrhythmias in Chagas disease

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The utilization of advance telemetry to investigate critical physiological parameters including electroencephalography in cynomolgus macaques following aerosol challenge with eastern equine encephalitis virus

John C. Trefry, Franco D. Rossi, Michael V. Accardi, Brandi L. Dorsey, Thomas R. Sprague, Suzanne E. Wollen-Roberts, Joshua D. Shamblin, Adrienne E. Kimmel, Pamela J. Glass, Lynn J. Miller, Crystal W. Burke, Anthony P. Cardile, Darci R. Smith, Sina Bavari, Simon Authier, William D. Pratt, Margaret L. Pitt, Farooq Nasar

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First description of Nodding Syndrome in the Central African Republic

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Eliminating onchocerciasis within the Meme River Basin of Cameroon: A social-ecological approach to understanding everyday realities and health systems

Theobald Mue Nji, Helen Piotrowski, Nnamdi Dum-Buo, Ebua Gallus Fung, Laura Dean, Sally Theobald, Rachael Thomson, Samuel Wanji, Kim Ozano

Cost of illness for severe and non-severe diarrhea borne by households in a low-income urban community of Bangladesh: A cross-sectional study

Rebeca Sultana, Stephen P. Luby, Emily S. Gurley, Nadia Ali Rimi, Sayeda Tasnuva Swarna, Jahangir A. M. Khan, Nazmun Nahar, Probir Kumar Ghosh, Sushil Ranjan Howlader, Humayun Kabir, Shifat Khan, Peter Kjær Mackie Jensen

Aedes aegypti SNAP and a calcium transporter ATPase influence dengue virus dissemination

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Evaluating spatiotemporal dynamics of snakebite in Sri Lanka: Monthly incidence mapping from a national representative survey sample

Dileepa Senajith Ediriweera, Anuradhani Kasthuriratne, Arunasalam Pathmeswaran, Nipul Kithsiri Gunawardene, Shaluka Francis Jayamanne, Kris Murray, Takuya Iwamura, Geoffrey Isbister, Andrew Dawson, David Griffith Lalloo, Hithanadura Janaka de Silva, Peter John Diggle

Individuals co-exposed to sand fly saliva and filarial parasites exhibit altered monocyte function

Moussa Sangare, Yaya Ibrahim Coulibaly, Naureen Huda, Silvia Vidal, Sameha Tariq, Michel Emmanuel Coulibaly, Siaka Yamoussa Coulibaly, Lamine Soumaoro, Ilo Dicko, Bourama Traore, Ibrahim Moussa Sissoko, Sekou Fantamady Traore, Ousmane Faye, Thomas B. Nutman, Jesus G. Valenzuela, Fabiano Oliveira, Seydou Doumbia, Shaden Kamhawi, Roshanak Tolouei Semnani

High incidence of asymptomatic cases during an outbreak of Plasmodium malariae in a remote village of Malaysian Borneo

Nurul Athirah Naserrudin, Emira Izzati Abdul Aziz, Erdie Aljet, George Mangunji, Bumpei Tojo, Mohammad Saffree Jeffree, Richard Culleton, Kamruddin Ahmed

Urogenital schistosomiasis infection prevalence targets to determine elimination as a public health problem based on microhematuria prevalence in school-age children

Ryan E. Wiegand, Fiona M. Fleming, Anne Straily, Susan P. Montgomery, Sake J. de Vlas, Jürg Utzinger, Penelope Vounatsou, W. Evan Secor

Serologic and molecular evidence for circulation of Crimean-Congo hemorrhagic fever virus in ticks and cattle in Zambia

Masahiro Kajihara, Martin Simuunza, Ngonda Saasa, George Dautu, Akina Mori-Kajihara, Yongjin Qiu, Ryo Nakao, Yoshiki Eto, Hayato Furumoto, Bernard M. Hang’ombe, Yasuko Orba, Hirofumi Sawa, Edgar Simulundu, Shuetsu Fukushi, Shigeru Morikawa, Masayuki Saijo, Jiro Arikawa, Swithine Kabilika, Mwaka Monze, Victor Mukonka, Aaron Mweene, Ayato Takada, Kumiko Yoshimatsu

Caregiver burden in Buruli ulcer disease: Evidence from Ghana

Yaw Ampem Amoako, Nancy Ackam, John-Paul Omuojine, Michael Ntiamoah Oppong, Abena Gyawu Owusu-Ansah, Mohammed Kabiru Abass, George Amofa, Elizabeth Ofori, Michael Frimpong, Freddie Bailey, David Hurst Molyneux, Richard Odame Phillips

Associations of IL13 gene polymorphisms and immune factors with Schistosoma haematobium infection in schoolchildren in four schistosomiasis-endemic communities in Ghana

Margaret Sarpong-Baidoo, Michael F. Ofori, Elias Kwesi Asuming-Brempong, Eric Kyei-Baafour, Bright K. Idun, Isaac Owusu-Frimpong, Nana A. Amonoo, Queenstar D. Quarshie, Edward J. Tettevi, Mike Y. Osei-Atweneboana

Undernutrition, food insecurity, and leprosy in North Gondar Zone, Ethiopia: A case-control study to identify infection risk factors associated with poverty

Puneet Anantharam, Lisa E. Emerson, Kassahun D. Bilcha, Jessica K. Fairley, Annisa B. Tesfaye

Determining seropositivity—A review of approaches to define population seroprevalence when using multiplex bead assays to assess burden of tropical diseases

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Detection and genetic characterisation of Toxoplasma gondii circulating in free-range chickens, pigs and seropositive pregnant women in Benue state, Nigeria

Ifeoma N. Nzelu, Jacob K. P. Kwaga, Junaidu Kabir, Idris A. Lawal, Christy Beazley, Laura Evans, Damer P. Blake

Correlation between reported dengue illness history and seropositivity in rural Thailand

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Evaluation of improved coloured targets to control riverine tsetse in East Africa: A Bayesian approach

Roger D. Santer, Michael N. Okal, Johan Esterhuizen, Steve J. Torr

Health and economic burden estimates of snakebite management upon health facilities in three regions of southern Burkina Faso

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Temperature, traveling, slums, and housing drive dengue transmission in a non-endemic metropolis

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Environmental characteristics around the household and their association with hookworm infection in rural communities from Bahir Dar, Amhara Region, Ethiopia

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Anti-chikungunya virus seroprevalence in Indigenous groups in the São Francisco Valley, Brazil

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A qualitative assessment of the context and enabling environment for the control of Taenia solium infections in endemic settings

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The role of let-7b in the inhibition of hepatic stellate cell activation by rSjP40

Xiaolei Sun, Li Zhang, Yuting Jiang, Aihong Li, Dandan Zhu, Jiangrong Wu, Yinong Duan

Immune exhaustion in chronic Chagas disease: Pro-inflammatory and immunomodulatory action of IL-27 in vitro

María Ailén Natale, Todd Minning, María Cecilia Albareda, Melisa Daiana Castro Eiro, María Gabriela Álvarez, Bruno Lococo, Gonzalo Cesar, Graciela Bertocchi, María Josefina Elias, María Belén Caputo, Rick Lee Tarleton, Susana Adriana Laucella

Characterization of a composite with enhanced attraction to savannah tsetse flies from constituents or analogues of tsetse refractory waterbuck (Kobus defassa) body odor

Benson M. Wachira, Joy M. Kabaka, Paul O. Mireji, Sylvance O. Okoth, Margaret M. Nganga, Robert Changasi, Patrick Obore, Bernard Ochieng’, Grace A. Murilla, Ahmed Hassanali

Short Report: Adult Aedes abundance and risk of dengue transmission

Janet Ong, Joel Aik, Lee Ching Ng

An integrated overview of the bacterial flora composition of Hyalomma anatolicum, the main vector of CCHF

Nayyereh Choubdar, Fateh Karimian, Mona Koosha, Mohammad Ali Oshaghi

Sensitivity and Specificity of two rapid tests for the diagnosis of infection by Trypanosoma cruzi in a Colombian population

Sandra Helena Suescún-Carrero, Lyda Pilar Salamanca-Cardozo, María-Jesus Pinazo, Lluis Armadans-Gil

An inactivated recombinant rabies virus displaying the Zika virus prM-E induces protective immunity against both pathogens

Hongli Jin, Cuicui Jiao, Zengguo Cao, Pei Huang, Hang Chi, Yujie Bai, Di Liu, Jianzhong Wang, Na Feng, Nan Li, Yongkun Zhao, Tiecheng Wang, Yuwei Gao, Songtao Yang, Xianzhu Xia, Hualei Wang

Short report: The potential of PCR on skin flakes from bed linens for diagnosis of scabies in an outbreak

Raïssa Tjon-Kon-Fat, Laurène Peckeu, Susan Hahné, Eric de Coster, Wendy Tas, Bas Wintermans, Anneke Bergmans, Mariska Petrignani, Ewout Fanoy

The synthetic synergistic cinnamon oil CIN-102 is active against Madurella mycetomatis, the most common causative agent of mycetoma

Mickey Konings, Kimberly Eadie, Wilson Lim, Ahmed H. Fahal, Johan Mouton, Nicolas Tesse, Wendy W. J. van de Sande

Stakeholders’ attitudes to outdoor residual spraying technique for dengue control in Malaysia: A PLS-SEM approach

Ahmad Firdhaus Arham, Latifah Amin, Muhammad Adzran Che Mustapa, Zurina Mahadi, Mashitoh Yaacob, Maznah Ibrahim

Orthobunyaviruses in the Caribbean: Melao and Oropouche virus infections in school children in Haiti in 2014

Maha A. Elbadry, Ricardo Durães-Carvalho, Gabriela M. Blohm, Caroline J. Stephenson, Julia C. Loeb, Sarah K. White, Taina Telisma, Sonese Chavannes, Valery M. Beau De Rochars, Marco Salemi, J. Glenn Morris Jr, John A. Lednicky

Active search strategies, clinicoimmunobiological determinants and training for implementation research confirm hidden endemic leprosy in inner São Paulo, Brazil

Fred Bernardes Filho, Claudia Maria Lincoln Silva, Glauber Voltan, Marcel Nani Leite, Ana Laura Rosifini Alves Rezende, Natália Aparecida de Paula, Josafá Gonçalves Barreto, Norma Tiraboschi Foss, Marco Andrey Cipriani Frade

Worldwide dynamic biogeography of zoonotic and anthroponotic dengue

Alisa Aliaga-Samanez, Marina Cobos-Mayo, Raimundo Real, Marina Segura, David Romero, Julia E. Fa, Jesús Olivero

Spatial distribution of Culex mosquito abundance and associated risk factors in Hanoi, Vietnam

Tuyen V. Ha, Wonkook Kim, Thang Nguyen-Tien, Johanna Lindahl, Hung Nguyen-Viet, Nguyen Quang Thi, Huy Van Nguyen, Fred Unger, Hu Suk Lee

Association between environmental and climatic risk factors and the spatial distribution of cystic and alveolar echinococcosis in Kyrgyzstan

Giulia Paternoster, Gianluca Boo, Roman Flury, Kursanbek M. Raimkulov, Gulnara Minbaeva, Jumagul Usubalieva, Maksym Bondarenko, Beat Müllhaupt, Peter Deplazes, Reinhard Furrer, Paul R. Torgerson

Lay attitudes and misconceptions and their implications for the control of brucellosis in an agro-pastoral community in Kilombero district, Tanzania

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Relative transmissibility of shigellosis among different age groups: A modeling study in Hubei Province, China

Zeyu Zhao, Qi Chen, Yao Wang, Meijie Chu, Qingqing Hu, Mikah Ngwanguong Hannah, Jia Rui, Xingchun Liu, Yunhan Yu, Fuwei Zhao, Zhengyun Ren, Shanshan Yu, Ran An, Lili Pan, Yi-Chen Chiang, Benhua Zhao, Yanhua Su, Bin Zhao, Tianmu Chen

Prevalence of bovine tuberculosis in dairy cattle in China during 2010–2019: A systematic review and meta-analysis

Qing-Long Gong, Yu Chen, Tian Tian, Xiaobo Wen, Dong Li, Yu-Hao Song, Qi Wang, Rui Du, Xiao-Xuan Zhang

Economic and disease burden of Japanese encephalitis in Zhejiang Province, 2013–2018

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Melioidosis–a disease of socioeconomic disadvantage

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A Listeria monocytogenes clone in human breast milk associated with severe acute malnutrition in West Africa: A multicentric case-controlled study

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