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November 2020

Bungarus multicinctus is the most venomous snake distributed in China and neighboring countries. LD50 values of isolated α-, β- and γ-bungarotoxins determined via three administration pathways indicated that the toxicities of β-bungarotoxin were closely related with injection routines but those of both α- and γ-bungarotoxins were not related. Commercial B. multicinctus antivenin weakly recognized α- and γ-bungarotoxins. Although B. multicinctus antivenin showed immunoreaction with high molecular weight fractions of B. fasciatus, Naja atra, Ophiophagus hannah venoms, the antivenin only demonstrated animal protection efficacy against O. hannah venom. Lin, et al. (2020)

Image Credit: Dr. Wen-Hui Lee

Editorial

A tribute to Cecilio Romaña: Romaña’s sign in Chagas disease

Nathan Beucler, Faustino Torrico, David Hibbert

Viewpoints

Policy Platform

Trypa-NO! contributes to the elimination of gambiense human African trypanosomiasis by combining tsetse control with “screen, diagnose and treat” using innovative tools and strategies

Joseph Mathu Ndung’u, Alain Boulangé, Albert Picado, Albert Mugenyi, Allan Mortensen, Andrew Hope, Brahim Guihini Mollo, Bruno Bucheton, Charles Wamboga, Charles Waiswa, Dramane Kaba, Enock Matovu, Fabrice Courtin, Gala Garrod, Geoffrey Gimonneau, Georgina V. Bingham, Hassane Mahamat Hassane, Inaki Tirados, Isabel Saldanha, Jacques Kabore, Jean-Baptiste Rayaisse, Jean-Mathieu Bart, Jessica Lingley, Johan Esterhuizen, Joshua Longbottom, Justin Pulford, Lingue Kouakou, Lassina Sanogo, Lucas Cunningham, Mamadou Camara, Mathurin Koffi, Michelle Stanton, Mike Lehane, Moise Saa Kagbadouno, Oumou Camara, Paul Bessell, Peka Mallaye, Philippe Solano, Richard Selby, Sophie Dunkley, Steve Torr, Sylvain Biéler, Veerle Lejon, Vincent Jamonneau, Wilfried Yoni, Zachary Katz

Research Articles

Comparing machine learning with case-control models to identify confirmed dengue cases

Tzong-Shiann Ho, Ting-Chia Weng, Jung-Der Wang, Hsieh-Cheng Han, Hao-Chien Cheng, Chun-Chieh Yang, Chih-Hen Yu, Yen-Jung Liu, Chien Hsiang Hu, Chun-Yu Huang, Ming-Hong Chen, Chwan-Chuen King, Yen-Jen Oyang, Ching-Chuan Liu

Circulating microtranscriptome profiles reveal distinct expression of microRNAs in severe leptospirosis

Umaporn Limothai, Janejira Dinhuzen, Titipon Payongsri, Sasipha Tachaboon, Pisit Tangkijvanich, Natthaya Chuaypen, Nattachai Srisawat

The risk of adverse clinical outcomes following treatment of Plasmodium vivax malaria with and without primaquine in Papua, Indonesia

Kamala Thriemer, Jeanne-Rini Poespoprodjo, Enny Kenangalem, Nicholas M. Douglas, Paulus Sugiarto, Nicholas M. Anstey, Julie Anne Simpson, Ric N. Price

Performance of molecular and serologic tests for the diagnosis of scrub typhus

Kavitha Kannan, Rebecca John, Debasree Kundu, Divya Dayanand, Kundavaram P. P. Abhilash, Alice Joan Mathuram, Anand Zachariah, Sowmya Sathyendra, Samuel G. Hansdak, O. C. Abraham, Karthik Gunasekaran, Ramya Iyadurai, Asha M. Abraham, John Antony Jude Prakash, Binesh Lal Yesudhason, Balaji Veeraraghavan, M. L. Kavitha, Linda R. Jose, M. N. Sumana, Kavitha Saravu, George M. Varghese

Morbidity management and surveillance of lymphatic filariasis disease and acute dermatolymphangioadenitis attacks using a mobile phone-based tool by community health volunteers in Ghana

Linda Batsa Debrah, Aliyu Mohammed, Jubin Osei-Mensah, Yusif Mubarik, Olivia Agbenyega, Nana Kwame Ayisi-Boateng, Kenneth Pfarr, Janina Melanie Kuehlwein, Ute Klarmann-Schulz, Achim Hoerauf, Alexander Yaw Debrah

A Bayesian phase 2 model based adaptive design to optimise antivenom dosing: Application to a dose-finding trial for a novel Russell’s viper antivenom in Myanmar

James A. Watson, Thomas Lamb, Jane Holmes, David A. Warrell, Khin Thida Thwin, Zaw Lynn Aung, Min Zaw Oo, Myat Thet Nwe, Frank Smithuis, Elizabeth A. Ashley

Long-read RNA sequencing of human and animal filarial parasites improves gene models and discovers operons

Nicolas J Wheeler, Paul M. Airs, Mostafa Zamanian

The effects of exposure to pyriproxyfen and predation on Zika virus infection and transmission in Aedes aegypti

Abdullah A. Alomar, Bradley H. Eastmond, Barry W. Alto

Antigenicity, stability, and reproducibility of Zika reporter virus particles for long-term applications

J. Charles Whitbeck, Anu Thomas, Kathryn Kadash-Edmondson, Ariadna Grinyo-Escuer, Lewis J. Stafford, Celine Cheng, Grant C. Liao, Frederick W. Holtsberg, M. Javad Aman, Graham Simmons, Edgar Davidson, Benjamin J. Doranz

Comparative analysis of virulence determinants, phylogroups, and antibiotic susceptibility patterns of typical versus atypical Enteroaggregative E. coli in India

Vinay Modgil, Jaspreet Mahindroo, Chandradeo Narayan, Manmohit Kalia, Md Yousuf, Varun Shahi, Meenakshi Koundal, Pankaj Chaudhary, Ruby Jain, Kawaljeet Singh Sandha, Seema Tanwar, Pratima Gupta, Kamlesh Thakur, Digvijay Singh, Neha Gautam, Manish Kakkar, Bhavneet Bharti, Balvinder Mohan, Neelam Taneja

West Nile virus in California, 2003–2018: A persistent threat

Robert E. Snyder, Tina Feiszli, Leslie Foss, Sharon Messenger, Ying Fang, Christopher M. Barker, William K. Reisen, Duc J. Vugia, Kerry A. Padgett, Vicki L. Kramer

Vaccination with novel low-molecular weight proteins secreted from Trichinella spiralis inhibits establishment of infection

Mellina T. Srey, Alessia Taccogna, Yelena Oksov, Sara Lustigman, Pei-Yi Tai, John Acord, Murray E. Selkirk, Tracey J. Lamb, David B. Guiliano

From resistance to persistence: Insights of a mathematical model on the indiscriminate use of insecticide

Helio Schechtman, Denise Valle, Max O. Souza

Spatial analysis of COVID-19 spread in Iran: Insights into geographical and structural transmission determinants at a province level

Ricardo Ramírez-Aldana, Juan Carlos Gomez-Verjan, Omar Yaxmehen Bello-Chavolla

Detection of the Nav channel kdr-like mutation and modeling of factors affecting survivorship of Culex quinquefasciatus mosquitoes from six areas of Harris County (Houston), Texas, after permethrin field-cage tests

Han-Jung Lee, Michael Longnecker, Travis L. Calkins, Andrew D. Renfro, Chris L. Fredregill, Mustapha Debboun, Patricia V. Pietrantonio

Immortalized stem cell-derived hepatocyte-like cells: An alternative model for studying dengue pathogenesis and therapy

Kessiri Kongmanas, Nuntaya Punyadee, Kasima Wasuworawong, Adisak Songjaeng, Tanapan Prommool, Yongyut Pewkliang, Siriphan Manocheewa, Somchai Thiemmeca, Khanit Sa-ngiamsuntorn, Chunya Puttikhunt, Kym Francis Faull, Suradej Hongeng, Panisadee Avirutnan

Modelling habitat suitability in Jordan for the cutaneous leishmaniasis vector (Phlebotomus papatasi) using multicriteria decision analysis

Emi A. Takahashi, Lina Masoud, Rami Mukbel, Javier Guitian, Kim B. Stevens

Linking mosquito surveillance to dengue fever through Bayesian mechanistic modeling

Clinton B. Leach, Jennifer A. Hoeting, Kim M. Pepin, Alvaro E. Eiras, Mevin B. Hooten, Colleen T. Webb

Salmonella identified in pigs in Kenya and Malawi reveals the potential for zoonotic transmission in emerging pork markets

Catherine N. Wilson, Caisey V. Pulford, James Akoko, Blanca Perez Sepulveda, Alexander V. Predeus, Jessica Bevington, Patricia Duncan, Neil Hall, Paul Wigley, Nicholas Feasey, Gina Pinchbeck, Jay C. D. Hinton, Melita A. Gordon, Eric M. Fèvre

Hazara virus and Crimean-Congo Hemorrhagic Fever Virus show a different pattern of entry in fully-polarized Caco-2 cell line

Vanessa Monteil, Cristiano Salata, Sofia Appelberg, Ali Mirazimi

A pilot study demonstrating the identification of Trypanosoma brucei gambiense and T. b. rhodesiense in vectors using a multiplexed high-resolution melt qPCR

Gala Garrod, Emily R. Adams, Jessica K. Lingley, Isabel Saldanha, Stephen J. Torr, Lucas J. Cunningham

Gender differences in the perceived need for community-wide deworming: Formative qualitative research from the DeWorm3 study, India

Kumudha Aruldas, Arianna Rubin Means, Angelin Titus, Yesudoss Jacob, Rajeshkumar Rajendiran, Jabaselvi Johnson, Mira Emmanuel-Fabula, Saravanakumar Puthupalayam Kaliappan, Sanjay Kamlakar Juvekar, Gagandeep Kang, Judd L. Walson, Sitara Swarna Rao Ajjampur

Mass administration of medicines in changing contexts: Acceptability, adaptability and community directed approaches in Kaduna and Ogun States, Nigeria

Oluwatosin Adekeye, Kim Ozano, Ruth Dixon, Elisabeth Osim Elhassan, Luret Lar, Elena Schmidt, Sunday Isiyaku, Okefu Okoko, Rachael Thomson, Sally Theobald, Laura Dean

Population genomic evidence that human and animal infections in Africa come from the same populations of Dracunculus medinensis

Caroline Durrant, Elizabeth A. Thiele, Nancy Holroyd, Stephen R. Doyle, Guillaume Sallé, Alan Tracey, Geetha Sankaranarayanan, Magda E. Lotkowska, Hayley M. Bennett, Thomas Huckvale, Zahra Abdellah, Ouakou Tchindebet, Mesfin Wossen, Makoy Samuel Yibi Logora, Cheick Oumar Coulibaly, Adam Weiss, Albrecht I. Schulte-Hostedde, Jeremy M. Foster, Christopher A. Cleveland, Michael J. Yabsley, Ernesto Ruiz-Tiben, Matthew Berriman, Mark L. Eberhard, James A. Cotton

SARS-CoV-2 lineage B.6 was the major contributor to early pandemic transmission in Malaysia

Yoong Min Chong, I-Ching Sam, Jennifer Chong, Maria Kahar Bador, Sasheela Ponnampalavanar, Sharifah Faridah Syed Omar, Adeeba Kamarulzaman, Vijayan Munusamy, Chee Kuan Wong, Fadhil Hadi Jamaluddin, Yoke Fun Chan

Hantavirus pulmonary syndrome outbreaks associated with climate variability in Northwestern Argentina, 1997–2017

Ignacio Ferro, Carla M. Bellomo, Walter López, Rocío Coelho, Daniel Alonso, Agostina Bruno, Francisco E. Córdoba, Valeria P. Martinez

Knowledge of health workers on snakes and snakebite management and treatment seeking behavior of snakebite victims in Bhutan

Sunil Sapkota, Deb P. Pandey, Guru P. Dhakal, Dhan B. Gurung

Early impairment of myocardial deformation assessed by regional speckle-tracking echocardiography in the indeterminate form of Chagas disease without fibrosis detected by cardiac magnetic resonance

Minna Moreira Dias Romano, Henrique Turin Moreira, José Antônio Marin-Neto, Priscila Elias Baccelli, Fawaz Alenezi, Igor Klem, Benedito Carlos Maciel, Joseph Kisslo, André Schmidt, Eric J. Velazquez

Determinants of two-year mortality among HIV positive patients with Cryptococcal meningitis initiating standard antifungal treatment with or without adjunctive dexamethasone in Uganda

Jonathan Kitonsa, Rebecca Nsubuga, Yunia Mayanja, Julius Kiwanuka, Yofesi Nikweri, Martin Onyango, Zacchaeus Anywaine, Abu-Baker Ggayi, Freddie Mukasa Kibengo, Pontiano Kaleebu, Jeremy Day

The economic burden of dengue fever in the Kingdom of Saudi Arabia

Naeema A. Akbar, Abdullah M. Assiri, Omima I. Shabouni, Osama M. Alwafi, Rajaa Al-Raddadi, Mohamad H. Alzahrani, Esam I. Azhar, Ashraf Amir, Abdullah M. Aljiffri, Abdulhakeem O. Althaqafi

A community survey of coverage and adverse events following country-wide triple-drug mass drug administration for lymphatic filariasis elimination, Samoa 2018

Gabriela A. Willis, Helen J. Mayfield, Therese Kearns, Take Naseri, Robert Thomsen, Katherine Gass, Sarah Sheridan, Patricia M. Graves, Colleen L. Lau

Analyses of the performance of the Ebola virus disease alert management system in South Sudan: August 2018 to November 2019

Olushayo Oluseun Olu, Richard Lako, Sudhir Bunga, Kibebu Berta, Matthew Kol, Patrick Otim Ramadan, Caroline Ryan, Ifeanyi Udenweze, Argata Guracha Guyo, Ishata Conteh, Qudsia Huda, Malick Gai, Dina Saulo, Heather Papowitz, Henry John Gray, Alex Chimbaru, Kencho Wangdi, Steven M. Grube, Beth Tippett Barr, Joseph Francis Wamala

Immunoreactivity and neutralization study of Chinese Bungarus multicinctus antivenin and lab-prepared anti-bungarotoxin antisera towards purified bungarotoxins and snake venoms

Bo Lin, Jia-Rui Zhang, Hui-Juan Lu, Lin Zhao, Jing Chen, Hong-Fei Zhang, Xue-Song Wei, Liang-Yu Zhang, Xiao-Bing Wu, Wen-Hui Lee

Epilepsy surveillance in normocephalic children with and without prenatal Zika virus exposure

Karen Blackmon, Randall Waechter, Barbara Landon, Trevor Noël, Calum Macpherson, Tyhiesia Donald, Nikita Cudjoe, Roberta Evans, Kemi S. Burgen, Piumi Jayatilake, Vivian Oyegunle, Otto Pedraza, Samah Abdel Baki, Thomas Thesen, Dennis Dlugos, Geetha Chari, Archana A. Patel, Elysse N. Grossi-Soyster, Amy R. Krystosik, A. Desiree LaBeaud

Extracellular non-coding RNA signatures of the metacestode stage of Echinococcus multilocularis

María Eugenia Ancarola, Gabriel Lichtenstein, Johannes Herbig, Nancy Holroyd, Mara Mariconti, Enrico Brunetti, Matthew Berriman, Krystyna Albrecht, Antonio Marcilla, Mara Cecilia Rosenzvit, Laura Kamenetzky, Klaus Brehm, Marcela Cucher

Evaluation of the sensitivity and specificity of GST-tagged recombinant antigens 2B2t, Ag5t and DIPOL in ELISA for the diagnosis and follow up of patients with cystic echinococcosis

Carlos Sánchez-Ovejero, Eylem Akdur, Raúl Manzano-Román, Ana Hernández-González, María González-Sánchez, David Becerro-Recio, Javier González-Miguel, Okan Akhan, Carmen M. Cretu, Kamenna Vutova, Francesca Tamarozzi, Mara Mariconti, Enrico Brunetti, Ambra Vola, Massimo Fabiani, Adriano Casulli, Mar Siles-Lucas

Broad diversity of Mycobacterium tuberculosis complex strains isolated from humans and cattle in Northern Algeria suggests a zoonotic transmission cycle

Hanane Damene, Djamel Tahir, Maren Diels, Ali Berber, Naima Sahraoui, Leen Rigouts

Time delays in treatment of snakebite patients in rural Sri Lanka and the need for rapid diagnostic tests

Anjana Silva, Jiri Hlusicka, Nipuna Siribaddana, Subodha Waiddyanatha, Senaka Pilapitiya, Prasanna Weerawansa, Niroshan Lokunarangoda, Sujeewa Thalgaspitiya, Sisira Siribaddana, Geoffrey K. Isbister

Transmission competence of a new mesonivirus, Yichang virus, in mosquitoes and its interference with representative flaviviruses

Guoguo Ye, Yujuan Wang, Xiaoyun Liu, Qiannan Dong, Quanxin Cai, Zhiming Yuan, Han Xia