Develop and Field Evolution of Single Tube Nested PCR, SYBRGreen PCR Methods, for the Diagnosis of Leprosy in Paraffin-embedded Formalin Fixed Tissues in Yunnan Province, a Hyper endemic Area of Leprosy in China

Xiaohua Chen; Yan Xing; Jun He; Fuyue Tan; Yuangang You; Yan Wen

doi:10.1371/journal.pntd.0007731

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Open Access

Peer-reviewed

Research Article

Develop and Field Evolution of Single Tube Nested PCR, SYBRGreen PCR Methods, for the Diagnosis of Leprosy in Paraffin-embedded Formalin Fixed Tissues in Yunnan Province, a Hyper endemic Area of Leprosy in China

Xiaohua Chen ,

Roles Data curation, Formal analysis, Writing – original draft, Writing – review & editing

* E-mail: hannahchen2003@163.com (XC); weny8@163.com (YW)

Affiliations Beijing Tropical Medicine Research Institute, Beijing Friendship Hospital, Capital Medical University, Beijing, China, Beijing Key Laboratory for Research on Prevention and Treatment of Tropical Diseases, Capital Medical University, Beijing, China
⨯
Yan Xing,

Roles Investigation, Methodology

Affiliations Beijing Tropical Medicine Research Institute, Beijing Friendship Hospital, Capital Medical University, Beijing, China, Beijing Key Laboratory for Research on Prevention and Treatment of Tropical Diseases, Capital Medical University, Beijing, China
⨯
Jun He,

Roles Investigation, Resources

Affiliation The Centers for Disease Control and Prevention of Yunnan Province, Kunming, China
⨯
Fuyue Tan,

Roles Investigation, Resources

Affiliation Wenshan Institute of Dermatology, Wenshan Dermatology Hospital, The Alliance Hospital of The First Affiliated Hospital of Kunming Medical University, Yunnan, China
⨯
Yuangang You,

Roles Investigation

Affiliations Beijing Tropical Medicine Research Institute, Beijing Friendship Hospital, Capital Medical University, Beijing, China, Beijing Key Laboratory for Research on Prevention and Treatment of Tropical Diseases, Capital Medical University, Beijing, China
⨯
Yan Wen

Roles Project administration, Supervision

* E-mail: hannahchen2003@163.com (XC); weny8@163.com (YW)

Affiliations Beijing Tropical Medicine Research Institute, Beijing Friendship Hospital, Capital Medical University, Beijing, China, Beijing Key Laboratory for Research on Prevention and Treatment of Tropical Diseases, Capital Medical University, Beijing, China

http://orcid.org/0000-0002-5792-6299

⨯

Develop and Field Evolution of Single Tube Nested PCR, SYBRGreen PCR Methods, for the Diagnosis of Leprosy in Paraffin-embedded Formalin Fixed Tissues in Yunnan Province, a Hyper endemic Area of Leprosy in China

Xiaohua Chen,
Yan Xing,
Jun He,
Fuyue Tan,
Yuangang You,
Yan Wen

Published: October 2, 2019
https://doi.org/10.1371/journal.pntd.0007731

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Polymerase chain reaction
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Leprosy
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