About the Authors
- Emily R Troemel
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Affiliations Department of Genetics, Harvard Medical School, Boston, Massachusetts, United States of America , Department of Molecular Biology, Massachusetts General Hospital, Boston, Massachusetts, United States of America
- Stephanie W Chu
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Affiliation Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts, United States of America
- Valerie Reinke
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Affiliation Department of Genetics, Yale University School of Medicine, New Haven, Connecticut, United States of America
- Siu Sylvia Lee
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Contributed equally to this work with: Siu Sylvia Lee, Frederick M Ausubel, Dennis H Kim
Affiliation Department of Molecular Biology and Genetics, Cornell University, Ithaca, New York, United States of America
- Frederick M Ausubel
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Contributed equally to this work with: Siu Sylvia Lee, Frederick M Ausubel, Dennis H Kim
Affiliations Department of Genetics, Harvard Medical School, Boston, Massachusetts, United States of America , Department of Molecular Biology, Massachusetts General Hospital, Boston, Massachusetts, United States of America
- Dennis H Kim
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Contributed equally to this work with: Siu Sylvia Lee, Frederick M Ausubel, Dennis H Kim
*To whom correspondence should be addressed. E-mail: dhkim@mit.edu
Affiliation Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts, United States of America
Competing Interests
The authors have declared that no competing interests exist.
Author Contributions
ERT, SSL, FMA, and DHK conceived and designed the experiments. ERT, SWC, VR, SSL, and DHK performed the experiments. ERT, SSL, and DHK analyzed the data. ERT, SSL, FMA, and DHK wrote the paper. SSL and DHK carried out initial pathogenesis and longevity experiments with sek-1, and VR and DHK carried out initial microarray analysis of sek-1–regulated genes. ERT carried out all subsequent pathogenesis assays with pmk-1; microarray and qRT-PCR analysis of pmk-1, daf-16, and pathogen-treated worms. SSL performed all lifespan experiments and associated statistical analysis. SWC carried out RNAi analysis of PMK-1–regulated genes.