March 2020

Shedding of cilia occurs from unicellular organisms to metazoans. Although essential during the cell cycle and during neurogenesis, the process remains poorly understood. In all cellular models, this phenomenon occurs distal to the transition zone (TZ), suggesting the underlying molecular mechanisms might be conserved. TZ module proteins are known to cooperate to establish TZ formation and function; to determine whether they also control deciliation, Gogendeau et al. studied the function of five TZ module proteins in the ciliate Paramecium tetraurelia. They found that all proteins are recruited to the TZ of growing cilia and localize with 9-fold symmetry at the level of the most distal part of the TZ. Depletion of some of these proteins induces constant deciliation of some cilia, while depletion of the others prevents calcium/ethanol-induced deciliation. This constitutes the first evidence for a role of conserved TZ proteins in deciliation and opens up new directions for understanding the physiology of motile cilia. The image shows the ciliary pattern of paramecia treated with control RNAi (top left) or RNAi for three different TZ proteins. Cells were stained for mono-glycylated tubulin (magenta) to show cilia tips and poly-glutamylated tubulin (green) to show basal bodies and cilia.

Image Credit: pbio.3000640

Editorial

The future of PLOS Biology

Nonia Pariente, on behalf of the PLOS Biology Staff Editors

PLOS Biology: published March 31, 2020 | https://doi.org/10.1371/journal.pbio.3000707

Essay

Vocal learning: Beyond the continuum

Pedro Tiago Martins, Cedric Boeckx

PLOS Biology: published March 30, 2020 | https://doi.org/10.1371/journal.pbio.3000672

Unsolved Mystery

Sex and suicide: The curious case of Toll-like receptors

Paulo A. Navarro-Costa, Antoine Molaro, Chandra S. Misra, Colin D. Meiklejohn, Peter J. Ellis

PLOS Biology: published March 23, 2020 | https://doi.org/10.1371/journal.pbio.3000663

Perspectives

Combating antiscience: Are we preparing for the 2020s?

Peter J. Hotez

PLOS Biology: published March 27, 2020 | https://doi.org/10.1371/journal.pbio.3000683

What is replication?

Brian A. Nosek, Timothy M. Errington

PLOS Biology: published March 27, 2020 | https://doi.org/10.1371/journal.pbio.3000691

Frontloading selectivity: A third way in scientific publishing?

Christopher D. Chambers

PLOS Biology: published March 25, 2020 | https://doi.org/10.1371/journal.pbio.3000693

Preregistration of exploratory research: Learning from the golden age of discovery

Ulrich Dirnagl

PLOS Biology: published March 26, 2020 | https://doi.org/10.1371/journal.pbio.3000690

Community Page

Interactive youth science workshops benefit student participants and graduate student mentors

Pallavi Kompella, Brant Gracia, Lucy LeBlanc, Shelly Engelman, Chinmayee Kulkarni, Niral Desai, Viviana June, Stephen March, Sarah Pattengale, Gabriel Rodriguez-Rivera, Seung Woo Ryu, Isabel Strohkendl, Pooja Mandke, Greg Clark

PLOS Biology: published March 30, 2020 | https://doi.org/10.1371/journal.pbio.3000668

Research Articles

Eco-evolutionary significance of “loners”

Fernando W. Rossine, Ricardo Martinez-Garcia, Allyson E. Sgro, Thomas Gregor, Corina E. Tarnita

PLOS Biology: published March 19, 2020 | https://doi.org/10.1371/journal.pbio.3000642

Context-dependent genetic architecture of Drosophila life span

Wen Huang, Terry Campbell, Mary Anna Carbone, W. Elizabeth Jones, Desiree Unselt, Robert R. H. Anholt, Trudy F. C. Mackay

PLOS Biology: published March 5, 2020 | https://doi.org/10.1371/journal.pbio.3000645

Evolutionary repair: Changes in multiple functional modules allow meiotic cohesin to support mitosis

Yu-Ying Phoebe Hsieh, Vasso Makrantoni, Daniel Robertson, Adèle L. Marston, Andrew W. Murray

PLOS Biology: published March 10, 2020 | https://doi.org/10.1371/journal.pbio.3000635

Context effects on probability estimation

Wei-Hsiang Lin, Justin L. Gardner, Shih-Wei Wu

PLOS Biology: published March 5, 2020 | https://doi.org/10.1371/journal.pbio.3000634

Neural oscillations in the fronto-striatal network predict vocal output in bats

Kristin Weineck, Francisco García-Rosales, Julio C. Hechavarría

PLOS Biology: published March 19, 2020 | https://doi.org/10.1371/journal.pbio.3000658

Early visual motion experience shapes the gap junction connections among direction selective ganglion cells

Li Zhang, Qiwen Wu, Yifeng Zhang

PLOS Biology: published March 25, 2020 | https://doi.org/10.1371/journal.pbio.3000692

Crumbs2 mediates ventricular layer remodelling to form the spinal cord central canal

Christine M. Tait, Kavitha Chinnaiya, Elizabeth Manning, Mariyam Murtaza, John-Paul Ashton, Nicholas Furley, Chris J. Hill, C. Henrique Alves, Jan Wijnholds, Kai S. Erdmann, Andrew Furley, Penny Rashbass, Raman M. Das, Kate G. Storey, Marysia Placzek

PLOS Biology: published March 9, 2020 | https://doi.org/10.1371/journal.pbio.3000470

CsIVP functions in vasculature development and downy mildew resistance in cucumber

Shuangshuang Yan, Kang Ning, Zhongyi Wang, Xiaofeng Liu, Yanting Zhong, Lian Ding, Hailing Zi, Zhihua Cheng, Xuexian Li, Hongyan Shan, Qingyang Lv, Laixin Luo, Renyi Liu, Liying Yan, Zhaoyang Zhou, William John Lucas, Xiaolan Zhang

PLOS Biology: published March 23, 2020 | https://doi.org/10.1371/journal.pbio.3000671

Eomes broadens the scope of CD8 T-cell memory by inhibiting apoptosis in cells of low affinity

Inga Kavazović, Hongya Han, Giulia Balzaretti, Erik Slinger, Niels A. W. Lemmermann, Anja ten Brinke, Doron Merkler, Jan Koster, Yenan T. Bryceson, Niek de Vries, Stipan Jonjić, Paul L. Klarenbeek, Bojan Polić, Eric Eldering, Felix M. Wensveen

PLOS Biology: published March 17, 2020 | https://doi.org/10.1371/journal.pbio.3000648

Swimming motility of a gut bacterial symbiont promotes resistance to intestinal expulsion and enhances inflammation

Travis J. Wiles, Brandon H. Schlomann, Elena S. Wall, Reina Betancourt, Raghuveer Parthasarathy, Karen Guillemin

PLOS Biology: published March 20, 2020 | https://doi.org/10.1371/journal.pbio.3000661

Drosophila-associated bacteria differentially shape the nutritional requirements of their host during juvenile growth

Jessika Consuegra, Théodore Grenier, Patrice Baa-Puyoulet, Isabelle Rahioui, Houssam Akherraz, Hugo Gervais, Nicolas Parisot, Pedro da Silva, Hubert Charles, Federica Calevro, François Leulier

PLOS Biology: published March 20, 2020 | https://doi.org/10.1371/journal.pbio.3000681

Bacterially produced metabolites protect C. elegans neurons from degeneration

Arles Urrutia, Víctor A. García-Angulo, Andrés Fuentes, Mauricio Caneo, Marcela Legüe, Sebastián Urquiza, Scarlett E. Delgado, Juan Ugalde, Paula Burdisso, Andrea Calixto

PLOS Biology: published March 24, 2020 | https://doi.org/10.1371/journal.pbio.3000638

A long noncoding RNA sensitizes genotoxic treatment by attenuating ATM activation and homologous recombination repair in cancers

Kunming Zhao, Xingwen Wang, Xuting Xue, Li Li, Ying Hu

PLOS Biology: published March 23, 2020 | https://doi.org/10.1371/journal.pbio.3000666

Mechanism of cargo recognition by retromer-linked SNX-BAR proteins

Xin Yong, Lin Zhao, Wankun Deng, Hongbin Sun, Xue Zhou, Lejiao Mao, Wenfeng Hu, Xiaofei Shen, Qingxiang Sun, Daniel D. Billadeau, Yu Xue, Da Jia

PLOS Biology: published March 9, 2020 | https://doi.org/10.1371/journal.pbio.3000631

A G protein–coupled receptor mediates neuropeptide-induced oocyte maturation in the jellyfish Clytia

Gonzalo Quiroga Artigas, Pascal Lapébie, Lucas Leclère, Philipp Bauknecht, Julie Uveira, Sandra Chevalier, Gáspár Jékely, Tsuyoshi Momose, Evelyn Houliston

PLOS Biology: published March 3, 2020 | https://doi.org/10.1371/journal.pbio.3000614

Cryo-EM structures of the human PA200 and PA200-20S complex reveal regulation of proteasome gate opening and two PA200 apertures

Hongxin Guan, Youwang Wang, Ting Yu, Yini Huang, Mianhuan Li, Abdullah F. U. H. Saeed, Vanja Perčulija, Daliang Li, Jia Xiao, Dongmei Wang, Ping Zhu, Songying Ouyang

PLOS Biology: published March 5, 2020 | https://doi.org/10.1371/journal.pbio.3000654

Dynamics in the murine norovirus capsid revealed by high-resolution cryo-EM

Joseph S. Snowden, Daniel L. Hurdiss, Oluwapelumi O. Adeyemi, Neil A. Ranson, Morgan R. Herod, Nicola J. Stonehouse

PLOS Biology: published March 31, 2020 | https://doi.org/10.1371/journal.pbio.3000649

Characterization of a membrane binding loop leads to engineering botulinum neurotoxin B with improved therapeutic efficacy

Linxiang Yin, Geoffrey Masuyer, Sicai Zhang, Jie Zhang, Shin-Ichiro Miyashita, David Burgin, Laura Lovelock, Shu-Fen Coker, Tian-min Fu, Pål Stenmark, Min Dong

PLOS Biology: published March 17, 2020 | https://doi.org/10.1371/journal.pbio.3000618

Low mutational load and high mutation rate variation in gut commensal bacteria

Ricardo S. Ramiro, Paulo Durão, Claudia Bank, Isabel Gordo

PLOS Biology: published March 10, 2020 | https://doi.org/10.1371/journal.pbio.3000617

Drifting codes within a stable coding scheme for working memory

Michael J. Wolff, Janina Jochim, Elkan G. Akyürek, Timothy J. Buschman, Mark G. Stokes

PLOS Biology: published March 2, 2020 | https://doi.org/10.1371/journal.pbio.3000625

Asymmetric sampling in human auditory cortex reveals spectral processing hierarchy

Jérémy Giroud, Agnès Trébuchon, Daniele Schön, Patrick Marquis, Catherine Liegeois-Chauvel, David Poeppel, Benjamin Morillon

PLOS Biology: published March 2, 2020 | https://doi.org/10.1371/journal.pbio.3000207

Maternal obesity-induced endoplasmic reticulum stress causes metabolic alterations and abnormal hypothalamic development in the offspring

Soyoung Park, Alice Jang, Sebastien G. Bouret

PLOS Biology: published March 12, 2020 | https://doi.org/10.1371/journal.pbio.3000296

A widespread family of heat-resistant obscure (Hero) proteins protect against protein instability and aggregation

Kotaro Tsuboyama, Tatsuya Osaki, Eriko Matsuura-Suzuki, Hiroko Kozuka-Hata, Yuki Okada, Masaaki Oyama, Yoshiho Ikeuchi, Shintaro Iwasaki, Yukihide Tomari

PLOS Biology: published March 12, 2020 | https://doi.org/10.1371/journal.pbio.3000632

The receptor tyrosine kinase Ror is required for dendrite regeneration in Drosophila neurons

Derek M. R. Nye, Richard M. Albertson, Alexis T. Weiner, J. Ian Hertzler, Matthew Shorey, Deborah C. I. Goberdhan, Clive Wilson, Kevin A. Janes, Melissa M. Rolls

PLOS Biology: published March 12, 2020 | https://doi.org/10.1371/journal.pbio.3000657

Endosomal Wnt signaling proteins control microtubule nucleation in dendrites

Alexis T. Weiner, Dylan Y. Seebold, Pedro Torres-Gutierrez, Christin Folker, Rachel D. Swope, Gregory O. Kothe, Jessica G. Stoltz, Madeleine K. Zalenski, Christopher Kozlowski, Dylan J. Barbera, Mit A. Patel, Pankajam Thyagarajan, Matthew Shorey, Derek M. R. Nye, Matthew Keegan, Kana Behari, Song Song, Jeffrey D. Axelrod, Melissa M. Rolls

PLOS Biology: published March 12, 2020 | https://doi.org/10.1371/journal.pbio.3000647

Pathogen transmission from vaccinated hosts can cause dose-dependent reduction in virulence

Richard I. Bailey, Hans H. Cheng, Margo Chase-Topping, Jody K. Mays, Osvaldo Anacleto, John R. Dunn, Andrea Doeschl-Wilson

PLOS Biology: published March 5, 2020 | https://doi.org/10.1371/journal.pbio.3000619

Urolithin A exerts antiobesity effects through enhancing adipose tissue thermogenesis in mice

Bo Xia, Xiao Chen Shi, Bao Cai Xie, Meng Qing Zhu, Yan Chen, Xin Yi Chu, Guo He Cai, Min Liu, Shi Zhen Yang, Grant A. Mitchell, Wei Jun Pang, Jiang Wei Wu

PLOS Biology: published March 27, 2020 | https://doi.org/10.1371/journal.pbio.3000688

Short Reports

The smell of hunger: Norway rats provision social partners based on odour cues of need

Karin Schneeberger, Gregory Röder, Michael Taborsky

PLOS Biology: published March 24, 2020 | https://doi.org/10.1371/journal.pbio.3000628

Neuronal activity triggers uptake of hematopoietic extracellular vesicles in vivo

Ivan-Maximiliano Kur, Pierre-Hugues Prouvot, Ting Fu, Wei Fan, Felicia Müller-Braun, Avash Das, Saumya Das, Thomas Deller, Jochen Roeper, Albrecht Stroh, Stefan Momma

PLOS Biology: published March 16, 2020 | https://doi.org/10.1371/journal.pbio.3000643

MKS-NPHP module proteins control ciliary shedding at the transition zone

Delphine Gogendeau, Michel Lemullois, Pierrick Le Borgne, Manon Castelli, Anne Aubusson-Fleury, Olivier Arnaiz, Jean Cohen, Christine Vesque, Sylvie Schneider-Maunoury, Khaled Bouhouche, France Koll, Anne-Marie Tassin

PLOS Biology: published March 12, 2020 | https://doi.org/10.1371/journal.pbio.3000640

Methods and Resources

IL-23 signaling regulation of pro-inflammatory T-cell migration uncovered by phosphoproteomics

Candelas Álvarez-Salamero, Raquel Castillo-González, Gloria Pastor-Fernández, Isabel R. Mariblanca, Jesús Pino, Danay Cibrian, María N. Navarro

PLOS Biology: published March 23, 2020 | https://doi.org/10.1371/journal.pbio.3000646

Surfactant-enhanced DNA accessibility to nuclease accelerates phenotypic β-lactam antibiotic susceptibility testing of Neisseria gonorrhoeae

Emily S. Savela, Nathan G. Schoepp, Matthew M. Cooper, Justin C. Rolando, Jeffrey D. Klausner, Olusegun O. Soge, Rustem F. Ismagilov

PLOS Biology: published March 19, 2020 | https://doi.org/10.1371/journal.pbio.3000651

Differential DNA accessibility to polymerase enables 30-minute phenotypic β-lactam antibiotic susceptibility testing of carbapenem-resistant Enterobacteriaceae

Nathan G. Schoepp, Eric J. Liaw, Alexander Winnett, Emily S. Savela, Omai B. Garner, Rustem F. Ismagilov

PLOS Biology: published March 19, 2020 | https://doi.org/10.1371/journal.pbio.3000652

Single-cell in vivo imaging of cellular circadian oscillators in zebrafish

Haifang Wang, Zeyong Yang, Xingxing Li, Dengfeng Huang, Shuguang Yu, Jie He, Yuanhai Li, Jun Yan

PLOS Biology: published March 13, 2020 | https://doi.org/10.1371/journal.pbio.3000435

A compact Cas9 ortholog from Staphylococcus Auricularis (SauriCas9) expands the DNA targeting scope

Ziying Hu, Shuai Wang, Chengdong Zhang, Ning Gao, Miaomiao Li, Deqian Wang, Daqi Wang, Dong Liu, Huihui Liu, Sang-Ging Ong, Hongyan Wang, Yongming Wang

PLOS Biology: published March 30, 2020 | https://doi.org/10.1371/journal.pbio.3000686

Cover

MKS-NPHP module proteins control ciliary shedding at the transition zone

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