April 2019

Choanoflagellates are microscopic aquatic organisms that can alternate between single-celled and multicellular states, and sequencing of their genomes has revealed that choanoflagellates are the closest single-celled relatives of animals. Moreover, choanoflagellates are a form of 'collar cell–a cell type crowned by an array of finger-like microvilli and a single, whip-like flagellum. This cell type is also found throughout the animal kingdom; therefore, studying the structure of the choanoflagellate collar cell can shed light on how this cell type and animal multicellularity might have evolved. This study by Laundon et al. used electron microscopy to reconstruct in 3D the total subcellular composition of single-celled and multicellular choanoflagellates as well as the collar cells from a marine sponge, which represents an early-branching animal lineage. The image shows 3D serial ultrathin transmission electron microscopy reconstructions of single (upper row) and colonial (lower row) cells from the choanoflagellate Salpingoeca rosetta. Each row shows the cells with the plasma membrane rendered transparent (left), and after progressive removal of organelles (center, left).

Image Credit: pbio.3000226

Editorial

Broadening the scope of PLOS Biology: Short Reports and Methods and Resources

The PLOS Biology Staff Editors

PLOS Biology: published April 26, 2019 | https://doi.org/10.1371/journal.pbio.3000248

Essay

How host genetics dictates successful viral zoonosis

Cody J. Warren, Sara L. Sawyer

PLOS Biology: published April 19, 2019 | https://doi.org/10.1371/journal.pbio.3000217

Perspectives

Getting serious about the challenge of regulating germline gene therapy

Arthur Caplan

PLOS Biology: published April 30, 2019 | https://doi.org/10.1371/journal.pbio.3000223

Gene-edited babies: What went wrong and what could go wrong

Haoyi Wang, Hui Yang

PLOS Biology: published April 30, 2019 | https://doi.org/10.1371/journal.pbio.3000224

Exact replication: Foundation of science or game of chance?

Sophie K. Piper, Ulrike Grittner, Andre Rex, Nico Riedel, Felix Fischer, Robert Nadon, Bob Siegerink, Ulrich Dirnagl

PLOS Biology: published April 9, 2019 | https://doi.org/10.1371/journal.pbio.3000188

Publication reform to safeguard wildlife from researcher harm

Kate A. Field, Paul C. Paquet, Kyle Artelle, Gilbert Proulx, Ryan K. Brook, Chris T. Darimont

PLOS Biology: published April 11, 2019 | https://doi.org/10.1371/journal.pbio.3000193

Primers

Monoclonal antibodies point to Achilles’ heel in picornavirus capsid

Mihnea Bostina

PLOS Biology: published April 17, 2019 | https://doi.org/10.1371/journal.pbio.3000232

Epigenetic regulator G9a provides glucose as a sweet key to stress resistance

Kelsie R. S. Doering, Stefan Taubert

PLOS Biology: published April 19, 2019 | https://doi.org/10.1371/journal.pbio.3000236

An elucidation of over a century old enigma in genetics—Heterosis

Diddahally R. Govindaraju

PLOS Biology: published April 24, 2019 | https://doi.org/10.1371/journal.pbio.3000215

Thought-based interaction: Same data, same methods, different results?

Reinhold Scherer

PLOS Biology: published April 8, 2019 | https://doi.org/10.1371/journal.pbio.3000190

The “backdoor pathway” of androgen synthesis in human male sexual development

Walter L. Miller, Richard J. Auchus

PLOS Biology: published April 3, 2019 | https://doi.org/10.1371/journal.pbio.3000198

Signaling by adenosine receptors—Homeostatic or allostatic control?

Rodrigo A. Cunha

PLOS Biology: published April 5, 2019 | https://doi.org/10.1371/journal.pbio.3000213

Community Page

Request a woman scientist: A database for diversifying the public face of science

Elizabeth A. McCullagh, Katarzyna Nowak, Anne Pogoriler, Jessica L. Metcalf, Maryam Zaringhalam, T. Jane Zelikova

PLOS Biology: published April 23, 2019 | https://doi.org/10.1371/journal.pbio.3000212

Research Articles

Genome-wide sexually antagonistic variants reveal long-standing constraints on sexual dimorphism in fruit flies

Filip Ruzicka, Mark S. Hill, Tanya M. Pennell, Ilona Flis, Fiona C. Ingleby, Richard Mott, Kevin Fowler, Edward H. Morrow, Max Reuter

PLOS Biology: published April 25, 2019 | https://doi.org/10.1371/journal.pbio.3000244

Nonlinear phenotypic variation uncovers the emergence of heterosis in Arabidopsis thaliana

François Vasseur, Louise Fouqueau, Dominique de Vienne, Thibault Nidelet, Cyrille Violle, Detlef Weigel

PLOS Biology: published April 24, 2019 | https://doi.org/10.1371/journal.pbio.3000214

The architecture of intra-organism mutation rate variation in plants

Long Wang, Yilun Ji, Yingwen Hu, Huaying Hu, Xianqin Jia, Mengmeng Jiang, Xiaohui Zhang, Lina Zhao, Yanchun Zhang, Yanxiao Jia, Chao Qin, Luyao Yu, Ju Huang, Sihai Yang, Laurence D. Hurst, Dacheng Tian

PLOS Biology: published April 9, 2019 | https://doi.org/10.1371/journal.pbio.3000191

To integrate or not to integrate: Temporal dynamics of hierarchical Bayesian causal inference

Máté Aller, Uta Noppeney

PLOS Biology: published April 2, 2019 | https://doi.org/10.1371/journal.pbio.3000210

Task-related hemodynamic responses are modulated by reward and task engagement

Mariana M. B. Cardoso, Bruss Lima, Yevgeniy B. Sirotin, Aniruddha Das

PLOS Biology: published April 19, 2019 | https://doi.org/10.1371/journal.pbio.3000080

Expectation and attention increase the integration of top-down and bottom-up signals in perception through different pathways

Noam Gordon, Naotsugu Tsuchiya, Roger Koenig-Robert, Jakob Hohwy

PLOS Biology: published April 30, 2019 | https://doi.org/10.1371/journal.pbio.3000233

Item-specific delay activity demonstrates concurrent storage of multiple active neural representations in working memory

David W. Sutterer, Joshua J. Foster, Kirsten C. S. Adam, Edward K. Vogel, Edward Awh

PLOS Biology: published April 26, 2019 | https://doi.org/10.1371/journal.pbio.3000239

The amplitude in periodic neural state trajectories underlies the tempo of rhythmic tapping

Jorge Gámez, Germán Mendoza, Luis Prado, Abraham Betancourt, Hugo Merchant

PLOS Biology: published April 8, 2019 | https://doi.org/10.1371/journal.pbio.3000054

Oxytocin blocks enhanced motivation for alcohol in alcohol dependence and blocks alcohol effects on GABAergic transmission in the central amygdala

Brendan J. Tunstall, Dean Kirson, Lia J. Zallar, Sam A. McConnell, Janaina C. M. Vendruscolo, Chelsea P. Ho, Christopher S. Oleata, Sophia Khom, Maurice Manning, Mary R. Lee, Lorenzo Leggio, George F. Koob, Marisa Roberto, Leandro F. Vendruscolo

PLOS Biology: published April 16, 2019 | https://doi.org/10.1371/journal.pbio.2006421

Glutamatergic neurons of the gigantocellular reticular nucleus shape locomotor pattern and rhythm in the freely behaving mouse

Maxime Lemieux, Frederic Bretzner

PLOS Biology: published April 24, 2019 | https://doi.org/10.1371/journal.pbio.2003880

Novel hybrid action of GABA mediates inhibitory feedback in the mammalian retina

James C. R. Grove, Arlene A. Hirano, Janira de los Santos, Cyrus F. McHugh, Shashvat Purohit, Greg D. Field, Nicholas C. Brecha, Steven Barnes

PLOS Biology: published April 1, 2019 | https://doi.org/10.1371/journal.pbio.3000200

Fine-tuned adaptation of embryo–endometrium pairs at implantation revealed by transcriptome analyses in Bos taurus

Fernando H. Biase, Isabelle Hue, Sarah E. Dickinson, Florence Jaffrezic, Denis Laloe, Harris A. Lewin, Olivier Sandra

PLOS Biology: published April 12, 2019 | https://doi.org/10.1371/journal.pbio.3000046

Regulation of the apical extension morphogenesis tunes the mechanosensory response of microvilliated neurons

Laura Desban, Andrew Prendergast, Julian Roussel, Marion Rosello, David Geny, Claire Wyart, Pierre-Luc Bardet

PLOS Biology: published April 19, 2019 | https://doi.org/10.1371/journal.pbio.3000235

Mouse screen reveals multiple new genes underlying mouse and human hearing loss

Neil J. Ingham, Selina A. Pearson, Valerie E. Vancollie, Victoria Rook, Morag A. Lewis, Jing Chen, Annalisa Buniello, Elisa Martelletti, Lorenzo Preite, Chi Chung Lam, Felix D. Weiss, Zӧe Powis, Pim Suwannarat, Christopher J. Lelliott, Sally J. Dawson, Jacqueline K. White, Karen P. Steel

PLOS Biology: published April 11, 2019 | https://doi.org/10.1371/journal.pbio.3000194

Infection-generated electric field in gut epithelium drives bidirectional migration of macrophages

Yaohui Sun, Brian Reid, Fernando Ferreira, Guillaume Luxardi, Li Ma, Kristen L. Lokken, Kan Zhu, Gege Xu, Yuxin Sun, Volodymyr Ryzhuk, Betty P. Guo, Carlito B. Lebrilla, Emanual Maverakis, Alex Mogilner, Min Zhao

PLOS Biology: published April 9, 2019 | https://doi.org/10.1371/journal.pbio.3000044

G1/S cell cycle regulators mediate effects of circadian dysregulation on tumor growth and provide targets for timed anticancer treatment

Yool Lee, Nicholas F. Lahens, Shirley Zhang, Joseph Bedont, Jeffrey M. Field, Amita Sehgal

PLOS Biology: published April 30, 2019 | https://doi.org/10.1371/journal.pbio.3000228

Feedforward regulation of Myc coordinates lineage-specific with housekeeping gene expression during B cell progenitor cell differentiation

Isabel Ferreirós-Vidal, Thomas Carroll, Tianyi Zhang, Vincenzo Lagani, Ricardo N. Ramirez, Elizabeth Ing-Simmons, Alicia G. Gómez-Valadés, Lee Cooper, Ziwei Liang, Georgios Papoutsoglou, Gopuraja Dharmalingam, Ya Guo, Sonia Tarazona, Sunjay J. Fernandes, Peri Noori, Gilad Silberberg, Amanda G. Fisher, Ioannis Tsamardinos, Ali Mortazavi, Boris Lenhard, Ana Conesa, Jesper Tegner, Matthias Merkenschlager, David Gomez-Cabrero

PLOS Biology: published April 12, 2019 | https://doi.org/10.1371/journal.pbio.2006506

Tubulin mRNA stability is sensitive to change in microtubule dynamics caused by multiple physiological and toxic cues

Ivana Gasic, Sarah A. Boswell, Timothy J. Mitchison

PLOS Biology: published April 9, 2019 | https://doi.org/10.1371/journal.pbio.3000225

Dhh1 promotes autophagy-related protein translation during nitrogen starvation

Xu Liu, Zhiyuan Yao, Meiyan Jin, Sim Namkoong, Zhangyuan Yin, Jun Hee Lee, Daniel J. Klionsky

PLOS Biology: published April 11, 2019 | https://doi.org/10.1371/journal.pbio.3000219

Up-regulation of FOXD1 by YAP alleviates senescence and osteoarthritis

Lina Fu, Yuqiong Hu, Moshi Song, Zunpeng Liu, Weiqi Zhang, Fa-Xing Yu, Jun Wu, Si Wang, Juan Carlos Izpisua Belmonte, Piu Chan, Jing Qu, Fuchou Tang, Guang-Hui Liu

PLOS Biology: published April 1, 2019 | https://doi.org/10.1371/journal.pbio.3000201

A novel evolutionary conserved mechanism of RNA stability regulates synexpression of primordial germ cell-specific genes prior to the sex-determination stage in medaka

Amaury Herpin, Cornelia Schmidt, Susanne Kneitz, Clara Gobé, Martina Regensburger, Aurélie Le Cam, Jérome Montfort, Mateus C. Adolfi, Christina Lillesaar, Dagmar Wilhelm, Michael Kraeussling, Brigitte Mourot, Béatrice Porcon, Maëlle Pannetier, Eric Pailhoux, Laurence Ettwiller, Dirk Dolle, Yann Guiguen, Manfred Schartl

PLOS Biology: published April 4, 2019 | https://doi.org/10.1371/journal.pbio.3000185

Distinct roles of two myosins in C. elegans spermatid differentiation

Junyan Hu, Shiya Cheng, Haibin Wang, Xin Li, Sun Liu, Mengmeng Wu, Yubing Liu, Xiaochen Wang

PLOS Biology: published April 16, 2019 | https://doi.org/10.1371/journal.pbio.3000211

Structure of the human ClC-1 chloride channel

Kaituo Wang, Sarah Spruce Preisler, Liying Zhang, Yanxiang Cui, Julie Winkel Missel, Christina Grønberg, Kamil Gotfryd, Erik Lindahl, Magnus Andersson, Kirstine Calloe, Pascal F. Egea, Dan Arne Klaerke, Michael Pusch, Per Amstrup Pedersen, Z. Hong Zhou, Pontus Gourdon

PLOS Biology: published April 25, 2019 | https://doi.org/10.1371/journal.pbio.3000218

Molecular understanding of calcium permeation through the open Orai channel

Xiaofen Liu, Guangyan Wu, Yi Yu, Xiaozhe Chen, Renci Ji, Jing Lu, Xin Li, Xing Zhang, Xue Yang, Yuequan Shen

PLOS Biology: published April 22, 2019 | https://doi.org/10.1371/journal.pbio.3000096

Structural basis for neutralization of hepatitis A virus informs a rational design of highly potent inhibitors

Lei Cao, Pi Liu, Pan Yang, Qiang Gao, Hong Li, Yao Sun, Ling Zhu, Jianping Lin, Dan Su, Zihe Rao, Xiangxi Wang

PLOS Biology: published April 30, 2019 | https://doi.org/10.1371/journal.pbio.3000229

A non-natural nucleotide uses a specific pocket to selectively inhibit telomerase activity

Wilnelly Hernandez-Sanchez, Wei Huang, Brian Plucinsky, Nelson Garcia-Vazquez, Nathaniel J. Robinson, William P. Schiemann, Anthony J. Berdis, Emmanuel Skordalakes, Derek J. Taylor

PLOS Biology: published April 5, 2019 | https://doi.org/10.1371/journal.pbio.3000204

Short Reports

The architecture of cell differentiation in choanoflagellates and sponge choanocytes

Davis Laundon, Ben T. Larson, Kent McDonald, Nicole King, Pawel Burkhardt

PLOS Biology: published April 12, 2019 | https://doi.org/10.1371/journal.pbio.3000226

TLR1/2 orchestrate human plasmacytoid predendritic cell response to gram+ bacteria

Salvatore Raieli, Coline Trichot, Sarantis Korniotis, Lucia Pattarini, Vassili Soumelis

PLOS Biology: published April 24, 2019 | https://doi.org/10.1371/journal.pbio.3000209

Methods and Resources

CRISPR screening using an expanded toolkit of autophagy reporters identifies TMEM41B as a novel autophagy factor

Christopher J. Shoemaker, Tina Q. Huang, Nicholas R. Weir, Nicole J. Polyakov, Sebastian W. Schultz, Vladimir Denic

PLOS Biology: published April 1, 2019 | https://doi.org/10.1371/journal.pbio.2007044

Meta-Research Article

Harmonization of resting-state functional MRI data across multiple imaging sites via the separation of site differences into sampling bias and measurement bias

Ayumu Yamashita, Noriaki Yahata, Takashi Itahashi, Giuseppe Lisi, Takashi Yamada, Naho Ichikawa, Masahiro Takamura, Yujiro Yoshihara, Akira Kunimatsu, Naohiro Okada, Hirotaka Yamagata, Koji Matsuo, Ryuichiro Hashimoto, Go Okada, Yuki Sakai, Jun Morimoto, Jin Narumoto, Yasuhiro Shimada, Kiyoto Kasai, Nobumasa Kato, Hidehiko Takahashi, Yasumasa Okamoto, Saori C. Tanaka, Mitsuo Kawato, Okito Yamashita, Hiroshi Imamizu

PLOS Biology: published April 18, 2019 | https://doi.org/10.1371/journal.pbio.3000042