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Correction: Plasmids manipulate bacterial behaviour through translational regulatory crosstalk

  • Catriona M. A. Thompson,
  • James P. J. Hall,
  • Govind Chandra,
  • Carlo Martins,
  • Gerhard Saalbach,
  • Supakan Panturat,
  • Susannah M. Bird,
  • Samuel Ford,
  • Richard H. Little,
  • Ainelen Piazza,
  • Ellie Harrison,
  • Robert W. Jackson,
  • Michael A. Brockhurst,
  • Jacob G. Malone
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The graph in Fig 8A was affected by a transposition error prior to publication. The authors have provided a corrected version here.

In the second paragraph of the Results sub-section ‘Carbon source sensing by RsmQ,’ the sixth sentence is incorrect. The correct sentence reads: Interestingly, cells carrying pQBR103Km –ΔrsmQ are able to metabolise citric acid and D-sorbitol at similar levels to WT cells, suggesting that RsmQ is able to repress metabolism of these carbon sources.

The third paragraph in the Results sub-section ‘Carbon source sensing by RsmQ’ should be disregarded.

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Fig 8. RsmQ is involved in the control of carbon source metabolism.

(a) Selected results from BioLog carbon source screens showing metabolism with relevant carbon sources after 48 h of static incubation at 28°C. Data are shown for SBW25 cells carrying pQBR103Km (red) and pQBR103Km-ΔrsmQ (blue) compared to average SBW25 plasmid free (green) for each carbon source. Error bars show standard deviation. A two-way ANOVA showed a significant effect of carbon source (p < 0.0001) as well as a significant interaction (p < 0.0001), but no effects of plasmid in this chosen dataset. Multiple comparisons compared SBW25 plasmid free, SBW25 +pQBR103Km and SBW25 +pQBR103Km- ΔrsmQ within each carbon source. Significant differences are indicated above for SBW25/SBW25 +pQBR103Km (a), SBW25/SBW25 +pQBR103Km-ΔrsmQ (b), and SBW25 +pQBR103Km /SBW25 +pQBR103Km-ΔrsmQ (c) for p < 0.05. Data for all carbon sources can be seen in S11 Fig and S2 Table. (b) Growth curves are shown for SBW25 (green), SBW25 cells carrying pQBR103Km (red), and pQBR103Km-ΔrsmQ (blue), with the mean growth for 3 biological replicates shown as a solid line and standard deviation shown as dotted lines. Cells were grown for 48 h at 28°C without shaking in M9 minimal media with 0.4% w/v either D-sorbitol, glycerol, L-alanine, citric acid, L-aspartic acid, L-histidine as indicated. Data are available in S7 Data. WT, wild-type.

https://doi.org/10.1371/journal.pbio.3002531.g001

Reference

  1. 1. Thompson CMA, Hall JPJ, Chandra G, Martins C, Saalbach G, Panturat S, et al. (2023) Plasmids manipulate bacterial behaviour through translational regulatory crosstalk. PLoS Biol 21(2): e3001988. pmid:36787297