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Open Access
Peer-reviewed
Methods and Resources
Methods and Resources report novel methods, substantial improvements to current methodologies, or informational datasets.
Efficient and error-free fluorescent gene tagging in human organoids without double-strand DNA cleavage
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Yannik Bollen ,
Contributed equally to this work with: Yannik Bollen, Joris H. Hageman
Roles Conceptualization, Formal analysis, Investigation, Methodology, Visualization, Writing – original draft, Writing – review & editing
Affiliations Molecular Cancer Research, Center for Molecular Medicine, University Medical Center Utrecht, Utrecht University, the Netherlands, Oncode Institute, Utrecht, the Netherlands, Medical Cell Biophysics, TechMed Centre, University of Twente, Enschede, the Netherlands
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Joris H. Hageman ,
Contributed equally to this work with: Yannik Bollen, Joris H. Hageman
Roles Formal analysis, Investigation, Methodology, Resources, Visualization, Writing – original draft, Writing – review & editing
Affiliations Molecular Cancer Research, Center for Molecular Medicine, University Medical Center Utrecht, Utrecht University, the Netherlands, Oncode Institute, Utrecht, the Netherlands
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Petra van Leenen,
Roles Formal analysis, Investigation, Visualization
Affiliations Molecular Cancer Research, Center for Molecular Medicine, University Medical Center Utrecht, Utrecht University, the Netherlands, Oncode Institute, Utrecht, the Netherlands
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Lucca L. M. Derks,
Roles Formal analysis, Investigation, Visualization, Writing – review & editing
Affiliations Oncode Institute, Utrecht, the Netherlands, Princess Máxima Center for Pediatric Oncology, Utrecht, the Netherlands
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Bas Ponsioen,
Roles Formal analysis, Visualization
Affiliations Molecular Cancer Research, Center for Molecular Medicine, University Medical Center Utrecht, Utrecht University, the Netherlands, Oncode Institute, Utrecht, the Netherlands
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Julian R. Buissant des Amorie,
Roles Methodology, Visualization, Writing – review & editing
Affiliations Molecular Cancer Research, Center for Molecular Medicine, University Medical Center Utrecht, Utrecht University, the Netherlands, Oncode Institute, Utrecht, the Netherlands
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Ingrid Verlaan-Klink,
Roles Investigation
Affiliations Molecular Cancer Research, Center for Molecular Medicine, University Medical Center Utrecht, Utrecht University, the Netherlands, Oncode Institute, Utrecht, the Netherlands
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Myrna van den Bos,
Roles Investigation
Affiliations Molecular Cancer Research, Center for Molecular Medicine, University Medical Center Utrecht, Utrecht University, the Netherlands, Oncode Institute, Utrecht, the Netherlands
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Leon W. M. M. Terstappen,
Roles Funding acquisition, Resources, Supervision
Affiliation Medical Cell Biophysics, TechMed Centre, University of Twente, Enschede, the Netherlands
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Ruben van Boxtel,
Roles Formal analysis, Supervision, Writing – review & editing
Affiliations Oncode Institute, Utrecht, the Netherlands, Princess Máxima Center for Pediatric Oncology, Utrecht, the Netherlands
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Hugo J. G. Snippert
Roles Conceptualization, Formal analysis, Funding acquisition, Methodology, Resources, Supervision, Visualization, Writing – original draft, Writing – review & editing
* E-mail: h.j.g.snippert@umcutrecht.nl
Affiliations Molecular Cancer Research, Center for Molecular Medicine, University Medical Center Utrecht, Utrecht University, the Netherlands, Oncode Institute, Utrecht, the Netherlands
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Efficient and error-free fluorescent gene tagging in human organoids without double-strand DNA cleavage
- Yannik Bollen,
- Joris H. Hageman,
- Petra van Leenen,
- Lucca L. M. Derks,
- Bas Ponsioen,
- Julian R. Buissant des Amorie,
- Ingrid Verlaan-Klink,
- Myrna van den Bos,
- Leon W. M. M. Terstappen,
- Ruben van Boxtel
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- Published: January 28, 2022
- https://doi.org/10.1371/journal.pbio.3001527