About the Authors
- Donghong Min
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Affiliation School of Computational Science, Florida State University, Tallahassee, Florida, United States of America
- Helen R Josephine
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Affiliation Department of Biochemistry, Brandeis University, Waltham, Massachusetts, United States of America
- Hongzhi Li
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Affiliation School of Computational Science, Florida State University, Tallahassee, Florida, United States of America
- Clemens Lakner
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Affiliation Department of Biological Sciences, Florida State University, Tallahassee, Florida, United States of America
- Iain S MacPherson
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Affiliation Department of Biochemistry, Brandeis University, Waltham, Massachusetts, United States of America
- Gavin J. P Naylor
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Affiliation Department of Biological Sciences, Florida State University, Tallahassee, Florida, United States of America
- David Swofford
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Affiliation Department of Biological Sciences, Florida State University, Tallahassee, Florida, United States of America
- Lizbeth Hedstrom
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*To whom correspondence should be addressed. E-mail: hedstrom@brandeis.edu (LH); yang@sb.fsu.edu (WY)
Affiliations Department of Biochemistry, Brandeis University, Waltham, Massachusetts, United States of America , Department of Chemistry, Brandeis University, Waltham, Massachusetts, United States of America
- Wei Yang
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*To whom correspondence should be addressed. E-mail: hedstrom@brandeis.edu (LH); yang@sb.fsu.edu (WY)
Affiliations School of Computational Science, Florida State University, Tallahassee, Florida, United States of America , Department of Chemistry and Biochemistry, Florida State University, Tallahassee, Florida, United States of America , Institute of Molecular Biophysics, Florida State University, Tallahassee, Florida, United States of America , College of Life Science, Nankai University, Tianjin 300071, China
Competing Interests
The authors have declared that no competing interests exist.
Author Contributions
DM and HL performed the simulations. HRJ performed and analyzed the pH dependence of the wild-type and mutant IMPDH reactions. ISM performed the complementation experiments. CL, GJPN, and DS performed and analyzed the phylogenetic analysis. LH and WY designed the study and wrote the paper. All authors discussed the results and commented on the paper.