PONE-D-24-28978Cloning of the DlERF10 gene from Diospyros lotus L. and cold resistance analysis of the DlERF10 gene in transgenic tobacco plantsPLOS ONE

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Reviewers' comments:

Reviewer's Responses to Questions

Comments to the Author

1. Is the manuscript technically sound, and do the data support the conclusions?

The manuscript must describe a technically sound piece of scientific research with data that supports the conclusions. Experiments must have been conducted rigorously, with appropriate controls, replication, and sample sizes. The conclusions must be drawn appropriately based on the data presented.

Reviewer #1: Yes

Reviewer #2: Yes

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2. Has the statistical analysis been performed appropriately and rigorously?

Reviewer #1: Yes

Reviewer #2: Yes

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3. Have the authors made all data underlying the findings in their manuscript fully available?

The PLOS Data policy requires authors to make all data underlying the findings described in their manuscript fully available without restriction, with rare exception (please refer to the Data Availability Statement in the manuscript PDF file). The data should be provided as part of the manuscript or its supporting information, or deposited to a public repository. For example, in addition to summary statistics, the data points behind means, medians and variance measures should be available. If there are restrictions on publicly sharing data—e.g. participant privacy or use of data from a third party—those must be specified.

Reviewer #1: Yes

Reviewer #2: Yes

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4. Is the manuscript presented in an intelligible fashion and written in standard English?

PLOS ONE does not copyedit accepted manuscripts, so the language in submitted articles must be clear, correct, and unambiguous. Any typographical or grammatical errors should be corrected at revision, so please note any specific errors here.

Reviewer #1: No

Reviewer #2: Yes

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5. Review Comments to the Author

Please use the space provided to explain your answers to the questions above. You may also include additional comments for the author, including concerns about dual publication, research ethics, or publication ethics. (Please upload your review as an attachment if it exceeds 20,000 characters)

Reviewer #1: Cloning of the DlERF10 gene from Diospyros lotus L. and cold resistance analysis of the DlERF10 gene in transgenic tobacco plants

Line 95: there is no appropriate reference for text: Xu et al. 2021

Line 237-239: there is no figure legend for figure 1

Line 241-242: what is second conserve DLERF10 protein (the figure 2 legend is ambiguous)

Line 314: what is the word and its number: proteins[45].

Line 351: figure 11 - there are no determined what figure is A, B or C

Line 488: please write correct the word: D. lous.

Line 497: has been demonstrated in previous studies.......what is previous study?

Line 536: The Gao YD et al. (2020) is no searchable

Line 562: The Wei et al (2020) is no searchable

This reference not used in the text:

Ohme-Takagi M, Shinshi H. Ethylene-inducible DNA binding proteins that interact764 with an ethylene-responsive element. The Plant Cell, 1995, 7(2): 173-182. DOI:765 10.1105/tpc.7.2.173.766

The discussion odf manuscript is very tall and boring for the readership

Sincerely yours

Bahram Kazemi PhD, Professor at Shahid Beheshti University of Medical Sciences

Reviewer #2: In this manuscript (PONE-D-24-28978) by Li, et al., nicely describes the Cloning of the DlERF10 gene from Diospyros lotus L. and cold resistance analysis of the DlERF10 gene in transgenic tobacco plants. Overall, I think this manuscript should be accepted after minor revision. but I only have a few minor concerns:

1.There are too many Figures in this manuscript, some figures should be placed together, thus the number of the figures could be set no more that 10.

2.There are many other transcription factors involved in abiotic stress responses, such as cold, salt and drought stress. So in this paper, more studies of WRKY, MYB, bHLH, or NAC in abiotic stress should be introduced in this MS.

3.More references of WRKY transcription factors in the regulation of biological or abiotic stress should be included in the revised manuscript, such as MbCBF2 (International Journal of Molecular Sciences. 2022, 23, 9827), MbERF12 (In Vitro Cellular & Developmental Biology - Plant. 2021, 57: 760–770) and MbWRKY4 (International  Journal of  Agriculture & Biology. 2018, 20: 2045–2052) from Malus baccata; MxWRKY55 (In Vitro Cellular & Developmental Biology - Plant. 2020, 56: 600–609), and MxWRKY53 (In Vitro Cellular & Developmental Biology - Plant. 2022, 58: 266–278) from Malus xiaojinensis; Furthermore, FvICE1 (Plant Physiology and Biochemistry. 2023, 196:270–280) from Fragaria vesca.

4.There should have bars in figure 12, and 14.

5.The Chinese characters should not appear in the figures.

6.Subcellular localization results should be added to the submission.

7.The expression levels of DlERF10 were studied under cold, drought and salt, why only the cold tolerance was researched?

8.The word “resistance” should changed into “ tolerance”.

9.If the figures were combined with more that 1 part, every part should be marked with A, B, C, and so on.

10.The quality of the written English should be improved. In this regard, you may request the assistance from a company specialized in scientific editing.

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Reviewer #1: No

Reviewer #2: No

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Attachments

Attachment

Submitted filename: Cloning of theDlERF10.pdf

Cloning of the DlERF10 gene from Diospyros lotus L. and cold resistance analysis of the DlERF10 gene in transgenic tobacco plants

PONE-D-24-28978R1

Dear Dr. Zhou,

We’re pleased to inform you that your manuscript has been judged scientifically suitable for publication and will be formally accepted for publication once it meets all outstanding technical requirements.

Within one week, you’ll receive an e-mail detailing the required amendments. When these have been addressed, you’ll receive a formal acceptance letter and your manuscript will be scheduled for publication.

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Kind regards,

Diaa Abd El-Moneim

Academic Editor

PLOS ONE

Additional Editor Comments (optional):

Reviewers' comments:

Reviewer's Responses to Questions

Comments to the Author

1. If the authors have adequately addressed your comments raised in a previous round of review and you feel that this manuscript is now acceptable for publication, you may indicate that here to bypass the “Comments to the Author” section, enter your conflict of interest statement in the “Confidential to Editor” section, and submit your "Accept" recommendation.

Reviewer #2: All comments have been addressed

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2. Is the manuscript technically sound, and do the data support the conclusions?

The manuscript must describe a technically sound piece of scientific research with data that supports the conclusions. Experiments must have been conducted rigorously, with appropriate controls, replication, and sample sizes. The conclusions must be drawn appropriately based on the data presented.

Reviewer #2: Yes

**********

3. Has the statistical analysis been performed appropriately and rigorously?

Reviewer #2: Yes

**********

4. Have the authors made all data underlying the findings in their manuscript fully available?

The PLOS Data policy requires authors to make all data underlying the findings described in their manuscript fully available without restriction, with rare exception (please refer to the Data Availability Statement in the manuscript PDF file). The data should be provided as part of the manuscript or its supporting information, or deposited to a public repository. For example, in addition to summary statistics, the data points behind means, medians and variance measures should be available. If there are restrictions on publicly sharing data—e.g. participant privacy or use of data from a third party—those must be specified.

Reviewer #2: Yes

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5. Is the manuscript presented in an intelligible fashion and written in standard English?

PLOS ONE does not copyedit accepted manuscripts, so the language in submitted articles must be clear, correct, and unambiguous. Any typographical or grammatical errors should be corrected at revision, so please note any specific errors here.

Reviewer #2: Yes

**********

6. Review Comments to the Author

Please use the space provided to explain your answers to the questions above. You may also include additional comments for the author, including concerns about dual publication, research ethics, or publication ethics. (Please upload your review as an attachment if it exceeds 20,000 characters)

Reviewer #2: In the revised manuccript, All my concerns have been resolved. So I think this manuscript can be accepted now.

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7. PLOS authors have the option to publish the peer review history of their article (what does this mean? ). If published, this will include your full peer review and any attached files.

If you choose “no”, your identity will remain anonymous but your review may still be made public.

Do you want your identity to be public for this peer review? For information about this choice, including consent withdrawal, please see our Privacy Policy .

Reviewer #2: Yes:  Deguo HAN

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