PONE-D-24-14273Effect of vegetable oil on ovarian steroidogenesis- A transcriptome approach to understand molecular mechanisms of hypothalamus pituitary and gonad axis in Ompok bimaculatusPLOS ONE

Dear Dr. Sundaray,

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 Specific comments by the academic editor are included in the attached filesThe quality of the figures need to be modified.

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Academic Editor

PLOS ONE

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Reviewers' comments:

Reviewer's Responses to Questions

Comments to the Author

1. Is the manuscript technically sound, and do the data support the conclusions?

The manuscript must describe a technically sound piece of scientific research with data that supports the conclusions. Experiments must have been conducted rigorously, with appropriate controls, replication, and sample sizes. The conclusions must be drawn appropriately based on the data presented.

Reviewer #1: Yes

Reviewer #2: Yes

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2. Has the statistical analysis been performed appropriately and rigorously?

Reviewer #1: I Don't Know

Reviewer #2: I Don't Know

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3. Have the authors made all data underlying the findings in their manuscript fully available?

The PLOS Data policy requires authors to make all data underlying the findings described in their manuscript fully available without restriction, with rare exception (please refer to the Data Availability Statement in the manuscript PDF file). The data should be provided as part of the manuscript or its supporting information, or deposited to a public repository. For example, in addition to summary statistics, the data points behind means, medians and variance measures should be available. If there are restrictions on publicly sharing data—e.g. participant privacy or use of data from a third party—those must be specified.

Reviewer #1: Yes

Reviewer #2: Yes

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4. Is the manuscript presented in an intelligible fashion and written in standard English?

PLOS ONE does not copyedit accepted manuscripts, so the language in submitted articles must be clear, correct, and unambiguous. Any typographical or grammatical errors should be corrected at revision, so please note any specific errors here.

Reviewer #1: No

Reviewer #2: No

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5. Review Comments to the Author

Please use the space provided to explain your answers to the questions above. You may also include additional comments for the author, including concerns about dual publication, research ethics, or publication ethics. (Please upload your review as an attachment if it exceeds 20,000 characters)

Reviewer #1: Thanks for your hard efforts. Please check the following comments

1- The abstract contains a lot of abbreviations that need to be rewritten fully (GSI, HIS, DEGs, HPG).

2- In the introduction section, the part between lines 58-66 needs to be rewritten to organize the aims of the study (please make the aims more direct)

3- In lines 76-77, what do you mean by; three tanks each in control and 77 treatment groups? Does that mean 3 tanks for each group?

4- What is the vegetable oil that has been used in this study? Mention its source and commercial data.

5- In lines 79-80 the mention of Table 1 here is not correct, it should be Table 3 instead. The table 1 should be in the results section. In addition, in the result section the author used Table 2 in describing the results of the fertilization rate and GSI that were described in Table 1, not Table 2.

6- In line 81 how did you know that the best treatment among these, i.e., T2

7- In line 88, what is the meaning of PTO and PCO?

8- How did you analyze the data statistically

9- In lines 155 – 156 please replace Table 2 with Table 1.

10- In line 181 Table 3 with Table 2.

11- In line 185 please replace the symptoms w3 and w6 with ω3 and ω6.

12- The discussion section needs to be rewritten (please do not repeat the results in the discussion)

Reviewer #2: Title:

Please modify Title; You have not addressed the source of vegetable oil in Abstract, and M&M. Please add this to The Title, Abstract, and M&M.

And in the title add HPG following Hypothalamus Pituitary Gonadal axis

Please modify "Jitendra Kumar Sundaray and Janmejay Parhi should be considered as joint corresponding author" to Jitendra Kumar Sundaray and Janmejay Parhi are equally responsible as corresponding Author.

Abstract:

Line 31 Please specify the source of vegetable oil

Line 33 Please indicate the full form of abbreviated words when the first appear in the text, i.e. GSI and HSI.

Introduction:

Line 42 change gonad to gonads

Line 43 change muscle to muscles

Line 50 change like to including

Line 56 change Fish to fish

Line 64 change So to therefore

M&M

Line 112 please revise and edit

Line 118 as above

Results

OK

Discussion:

Line 183 Please replace "depict" with an appropriate verb. Depict means represent by a drawing, painting, or other art form.

Again, Please indicate the source of vegetable oil.

Line 184 change "Several studies earlier describe" to Several earlier studies showed..

Line 184 Several studies earlier describe the beneficial effects of dietary lipid on

ovarian development (29-32). In which species?

Line 185 please correct omega symbol

Line 186 vitamins

Line 186 change gave a better result to gave a better understanding

Line 191 please revise. The writing style needs professional check.

Line 221 please revise

Line 267 can hence what?

Line 273 it can not

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Reviewer #1: No

Reviewer #2: Yes: Reza Masoumi, A/Prof of Animal Physiology

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Attachments

Attachment

Submitted filename: comment.docx

Attachment

Submitted filename: PONE-D-24-14273 (Editor).pdf

Effect of vegetable oil on ovarian steroidogenesis- A transcriptome approach to understand molecular mechanisms of hypothalamus pituitary and gonad axis (HPG) in Ompok bimaculatus

PONE-D-24-14273R1

Dear Dr. Jitendra Kumar,

We’re pleased to inform you that your manuscript has been judged scientifically suitable for publication and will be formally accepted for publication once it meets all outstanding technical requirements.

Within one week, you’ll receive an e-mail detailing the required amendments. When these have been addressed, you’ll receive a formal acceptance letter and your manuscript will be scheduled for publication.

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If your institution or institutions have a press office, please notify them about your upcoming paper to help maximize its impact. If they’ll be preparing press materials, please inform our press team as soon as possible -- no later than 48 hours after receiving the formal acceptance. Your manuscript will remain under strict press embargo until 2 pm Eastern Time on the date of publication. For more information, please contact onepress@plos.org.

Kind regards,

Amel Mohamed El Asely

Academic Editor

PLOS ONE

Additional Editor Comments (optional):

Reviewers' comments:

Reviewer's Responses to Questions

Comments to the Author

1. If the authors have adequately addressed your comments raised in a previous round of review and you feel that this manuscript is now acceptable for publication, you may indicate that here to bypass the “Comments to the Author” section, enter your conflict of interest statement in the “Confidential to Editor” section, and submit your "Accept" recommendation.

Reviewer #1: All comments have been addressed

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2. Is the manuscript technically sound, and do the data support the conclusions?

The manuscript must describe a technically sound piece of scientific research with data that supports the conclusions. Experiments must have been conducted rigorously, with appropriate controls, replication, and sample sizes. The conclusions must be drawn appropriately based on the data presented.

Reviewer #1: Yes

**********

3. Has the statistical analysis been performed appropriately and rigorously?

Reviewer #1: I Don't Know

**********

4. Have the authors made all data underlying the findings in their manuscript fully available?

The PLOS Data policy requires authors to make all data underlying the findings described in their manuscript fully available without restriction, with rare exception (please refer to the Data Availability Statement in the manuscript PDF file). The data should be provided as part of the manuscript or its supporting information, or deposited to a public repository. For example, in addition to summary statistics, the data points behind means, medians and variance measures should be available. If there are restrictions on publicly sharing data—e.g. participant privacy or use of data from a third party—those must be specified.

Reviewer #1: Yes

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5. Is the manuscript presented in an intelligible fashion and written in standard English?

PLOS ONE does not copyedit accepted manuscripts, so the language in submitted articles must be clear, correct, and unambiguous. Any typographical or grammatical errors should be corrected at revision, so please note any specific errors here.

Reviewer #1: Yes

**********

6. Review Comments to the Author

Please use the space provided to explain your answers to the questions above. You may also include additional comments for the author, including concerns about dual publication, research ethics, or publication ethics. (Please upload your review as an attachment if it exceeds 20,000 characters)

Reviewer #1: Thanks for the hard work. Your paper has a novel idea, good methodology, and well written. The reviewing items that I have asked are completely answered. Good luck

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7. PLOS authors have the option to publish the peer review history of their article (what does this mean?). If published, this will include your full peer review and any attached files.

If you choose “no”, your identity will remain anonymous but your review may still be made public.

Do you want your identity to be public for this peer review? For information about this choice, including consent withdrawal, please see our Privacy Policy.

Reviewer #1: Yes: AHMED ELKHAWAGAH

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