N-glycoproteome Analysis of the Secretome of Human Metastatic Hepatocellular Carcinoma Cell Lines Combining Hydrazide Chemistry, HILIC Enrichment and Mass Spectrometry

Xianyu Li; Jing Jiang; Xinyuan Zhao; Jifeng Wang; Huanhuan Han; Yan Zhao; Bo Peng; Rugang Zhong; Wantao Ying; Xiaohong Qian

doi:10.1371/journal.pone.0081921

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Open Access

Peer-reviewed

Research Article

N-glycoproteome Analysis of the Secretome of Human Metastatic Hepatocellular Carcinoma Cell Lines Combining Hydrazide Chemistry, HILIC Enrichment and Mass Spectrometry

Xianyu Li,

Affiliations College of Life Science and Bio-engineering, Beijing University of Technology, Beijing, China, State Key Laboratory of Proteomics, Beijing Proteome Research Center, Beijing Institute of Radiation Medicine, Beijing, China
⨯
Jing Jiang,

Affiliation State Key Laboratory of Proteomics, Beijing Proteome Research Center, Beijing Institute of Radiation Medicine, Beijing, China
⨯
Xinyuan Zhao,

Affiliations College of Life Science and Bio-engineering, Beijing University of Technology, Beijing, China, State Key Laboratory of Proteomics, Beijing Proteome Research Center, Beijing Institute of Radiation Medicine, Beijing, China
⨯
Jifeng Wang,

Affiliations College of Life Science and Bio-engineering, Beijing University of Technology, Beijing, China, State Key Laboratory of Proteomics, Beijing Proteome Research Center, Beijing Institute of Radiation Medicine, Beijing, China
⨯
Huanhuan Han,

Affiliation State Key Laboratory of Proteomics, Beijing Proteome Research Center, Beijing Institute of Radiation Medicine, Beijing, China
⨯
Yan Zhao,

Affiliation State Key Laboratory of Proteomics, Beijing Proteome Research Center, Beijing Institute of Radiation Medicine, Beijing, China
⨯
Bo Peng,

Affiliation State Key Laboratory of Proteomics, Beijing Proteome Research Center, Beijing Institute of Radiation Medicine, Beijing, China
⨯
Rugang Zhong,

Affiliation College of Life Science and Bio-engineering, Beijing University of Technology, Beijing, China
⨯
Wantao Ying ,

* E-mail: proteomics@126.com (WY); qianxh1@163.com (XQ)

Affiliation State Key Laboratory of Proteomics, Beijing Proteome Research Center, Beijing Institute of Radiation Medicine, Beijing, China
⨯
Xiaohong Qian

* E-mail: proteomics@126.com (WY); qianxh1@163.com (XQ)

Affiliations College of Life Science and Bio-engineering, Beijing University of Technology, Beijing, China, State Key Laboratory of Proteomics, Beijing Proteome Research Center, Beijing Institute of Radiation Medicine, Beijing, China
⨯

N-glycoproteome Analysis of the Secretome of Human Metastatic Hepatocellular Carcinoma Cell Lines Combining Hydrazide Chemistry, HILIC Enrichment and Mass Spectrometry

Xianyu Li,
Jing Jiang,
Xinyuan Zhao,
Jifeng Wang,
Huanhuan Han,
Yan Zhao,
Bo Peng,
Rugang Zhong,
Wantao Ying,
Xiaohong Qian

Published: December 4, 2013
https://doi.org/10.1371/journal.pone.0081921

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Metastasis
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Hepatocellular carcinoma
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Glycoproteins
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