Generation and Validation of a Shewanella oneidensis MR-1 Clone Set for Protein Expression and Phage Display

Haichun Gao; Donna Pattison; Tingfen Yan; Dawn M. Klingeman; Xiaohu Wang; Joseph Petrosino; Lisa Hemphill; Xiufeng Wan; Adam B. Leaphart; George M. Weinstock; Timothy Palzkill; Jizhong Zhou

doi:10.1371/journal.pone.0002983

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Open Access

Peer-reviewed

Research Article

Generation and Validation of a Shewanella oneidensis MR-1 Clone Set for Protein Expression and Phage Display

Haichun Gao ,

Contributed equally to this work with: Haichun Gao, Donna Pattison, Tingfen Yan, Dawn M. Klingeman

Affiliations Institute for Environmental Genomics, University of Oklahoma, Norman, Oklahoma, United States of America, Environmental Sciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee, United States of America
⨯
Donna Pattison ,

Contributed equally to this work with: Haichun Gao, Donna Pattison, Tingfen Yan, Dawn M. Klingeman

Affiliation Baylor College of Medicine, Houston, Texas, United States of America
⨯
Tingfen Yan ,

Contributed equally to this work with: Haichun Gao, Donna Pattison, Tingfen Yan, Dawn M. Klingeman

Affiliation Environmental Sciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee, United States of America
⨯
Dawn M. Klingeman ,

Contributed equally to this work with: Haichun Gao, Donna Pattison, Tingfen Yan, Dawn M. Klingeman

Affiliation Environmental Sciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee, United States of America
⨯
Xiaohu Wang,

Affiliation Baylor College of Medicine, Houston, Texas, United States of America
⨯
Joseph Petrosino,

Affiliation Baylor College of Medicine, Houston, Texas, United States of America
⨯
Lisa Hemphill,

Affiliation Baylor College of Medicine, Houston, Texas, United States of America
⨯
Xiufeng Wan,

Affiliation Environmental Sciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee, United States of America
⨯
Adam B. Leaphart,

Affiliation Environmental Sciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee, United States of America
⨯
George M. Weinstock,

Affiliation Baylor College of Medicine, Houston, Texas, United States of America
⨯
Timothy Palzkill ,

* E-mail: timothyp@bcm.tmc.edu (TP); jzhou@ou.edu (JZ)

Affiliation Baylor College of Medicine, Houston, Texas, United States of America
⨯
Jizhong Zhou

* E-mail: timothyp@bcm.tmc.edu (TP); jzhou@ou.edu (JZ)

Affiliations Institute for Environmental Genomics, University of Oklahoma, Norman, Oklahoma, United States of America, Environmental Sciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee, United States of America
⨯

Generation and Validation of a Shewanella oneidensis MR-1 Clone Set for Protein Expression and Phage Display

Haichun Gao,
Donna Pattison,
Tingfen Yan,
Dawn M. Klingeman,
Xiaohu Wang,
Joseph Petrosino,
Lisa Hemphill,
Xiufeng Wan,
Adam B. Leaphart,
George M. Weinstock

Published: August 20, 2008
https://doi.org/10.1371/journal.pone.0002983

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Cloning
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Phage display
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Protein expression
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Plasmid construction
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Gene prediction
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Polymerase chain reaction
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DNA cloning
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DNA-binding proteins
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